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Multiple stimulations for muscle–nerve–blood vessel unit in compensatory hypertrophied skeletal muscle of rat surgical ablation model

Abstract

Tissue inflammation and multiple cellular responses in the compensatory enlarged plantaris (OP Plt) muscle induced by surgical ablation of synergistic muscles (soleus and gastrocnemius) were followed over 10 weeks after surgery. Contralateral surgery was performed in adult Wistar male rats. Cellular responses in muscle fibers, blood vessels and nerve fibers were analyzed by immunohistochemistry and electron microscopy. Severe muscle fiber damage and disappearance of capillaries associated with apparent tissue edema were observed in the peripheral portion of OP Plt muscles during the first week, whereas central portions were relatively preserved. Marked cell activation/proliferation was also mainly observed in peripheral portions. Similarly, activated myogenic cells were seen not only inside but also outside of muscle fibers. The former were likely satellite cells and the latter may be interstitial myogenic cells. One week after surgery, small muscle fibers, small arteries and capillaries and several branched-muscle fibers were evident in the periphery, thus indicating new muscle fiber and blood vessel formation. Proliferating cells were also detected in the nerve bundles in the Schwann cell position. These results indicate that the compensatory stimulated/enlarged muscle is a suitable model for analyzing multiple physiological cellular responses in muscle–nerve–blood vessel units under continuous stretch stimulation.

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Acknowledgment

This work was supported by a Grant-in-Aid for Scientific Research (B-12480012) from the Ministry of Education, Science and Culture of Japan.

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Correspondence to Tetsuro Tamaki.

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418_2009_585_MOESM1_ESM.tif

Supplemental Figure 1. Basal lamina shape between large mature and small immature fibers presenting in Figure 5E. Individual basal lamina can be seen in both fibers (arrows). Satellite cells having small amounts of cytoplasm are also evident in large mature fibers (SC). Gaps in both fibers are also evident due to the periphery of unknown cells (asterisk). (TIFF 672 kb)

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Tamaki, T., Uchiyama, Y., Okada, Y. et al. Multiple stimulations for muscle–nerve–blood vessel unit in compensatory hypertrophied skeletal muscle of rat surgical ablation model. Histochem Cell Biol 132, 59–70 (2009). https://doi.org/10.1007/s00418-009-0585-1

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  • DOI: https://doi.org/10.1007/s00418-009-0585-1

Keywords

  • Muscle fiber regeneration
  • Muscle fiber hyperplasia
  • Vascular formation
  • Peripheral nerve cells
  • Branched-muscle fiber