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Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney

Abstract

The baboon is an ideal animal model to study human kidney development. The aim of the current study was to use immunohistochemistry to localise the antigens TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney where nephrogenesis was still on-going and in kidneys where nephrogenesis was complete. Fixed kidney sections from baboons delivered at 125, 140, 175 and 185 days gestation (term = 185 days) were immuno-labelled with antibodies directed against TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin. In kidneys with on-going nephrogenesis (125 and 140 days gestation), TRA-1-60, TRA-1-81 and GCTM-2 were specifically localised to the apical plasma membrane of the epithelium of the ureteric ampullae and the collecting ducts, while podocalyxin immunostaining was not detected. In kidneys where nephrogenesis was complete (175 and 185 days gestation) localisation of these markers was again very specifically localised to the collecting ducts. In conclusion, although further experimentation is required to confirm the identity of the specific cell types marked by these antibodies, this study provides new insight into the distribution of commonly utilised stem cell antibodies in the developing baboon kidney.

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Acknowledgments

We would like to thank the Southwest Foundation for Biomedical Research (San Antonio, TX) for provision of the baboon kidneys, in particular Jacqueline Coalson and Vicki Winter from the University of Texas Health Science Center for their assistance in obtaining the kidneys. This work was supported by NIH HL52636 BPD Resource Center grant and P51RR13986 for facility support.

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Correspondence to Lina Gubhaju.

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Gubhaju, L., Laslett, A., Bertram, J.F. et al. Immunohistochemical localisation of TRA-1-60, TRA-1-81, GCTM-2 and podocalyxin in the developing baboon kidney. Histochem Cell Biol 129, 651–657 (2008). https://doi.org/10.1007/s00418-008-0398-7

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  • DOI: https://doi.org/10.1007/s00418-008-0398-7

Keywords

  • Kidney
  • Nephrogenesis
  • Baboon
  • Stem cells