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The calcium-sensing protein synaptotagmin 7 is expressed on different endosomal compartments in endocrine, neuroendocrine cells or neurons but not on large dense core vesicles

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Abstract

Synaptotagmin (syt) isoforms function as calcium sensor in post-Golgi transport although the precise transport step and compartment(s) concerned are still not fully resolved. As syt7 has been proposed to operate in lysosomal exocytosis and in exocytosis of large dense core vesicles (LDCVs), we have addressed the distribution of endogenous syt7 in insulin-secreting cells. These cells express different syt7 isoforms comparable to neurons. According to subcellular fractionation and quantitative confocal immunocytochemistry, syt7 is not found on LDCVs or on synaptic-like microvesicles but colocalizes with Rab7 on endosomes and to structures near to or at the plasma membrane. Similarly, endogenous syt7 was absent from LDCVs in pheochromocytoma PC12 cells. In contrast, syt7 localised to lysosomes in both, PC12 cells and hippocampal neurons. In conclusion, endogenous syt7 shows a wider distribution than previously reported but does not qualify as vesicular calcium sensor in SLMV or LDCV exocytosis according to its localisation.

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Acknowledgments

We thank Delphine Bouchet for excellent technical assistance. This work was supported by grants from the National Ministry of Research, the Region of Aquitaine and the University of Bordeaux I (BQR2004/5).

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Authors and Affiliations

  1. Institut Européen de Chimie et Biologie, Université de Bordeaux 1, JE 2390, 33607, Pessac/Bordeaux, France

    Carole Monterrat, Florence Grise, Marie Noëlle Benassy & Jochen Lang

  2. Université Bordeaux 2, Physiologie Cellulaire de la Synapse UMR 5091, Bordeaux, France

    Agnès Hémar

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  1. Carole Monterrat
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  2. Florence Grise
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  3. Marie Noëlle Benassy
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  4. Agnès Hémar
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  5. Jochen Lang
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Correspondence to Jochen Lang.

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Monterrat, C., Grise, F., Benassy, M.N. et al. The calcium-sensing protein synaptotagmin 7 is expressed on different endosomal compartments in endocrine, neuroendocrine cells or neurons but not on large dense core vesicles. Histochem Cell Biol 127, 625–632 (2007). https://doi.org/10.1007/s00418-007-0271-0

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