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Regulation of Toll-like receptor 4 expression in mouse colon by macrophage migration inhibitory factor

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An Erratum to this article was published on 08 December 2005

An Erratum to this article was published on 08 December 2005

Abstract

Recent studies have indicated that macrophage migration inhibitory factor (MIF) and Toll-like receptor (TLR) play an important role in the regulation of innate immune responses. In this study, we investigated the effect of MIF on the expression of TLR4, a receptor that recognizes lipopolysaccharide, in colon using MIF-deficient mice. TLR4 mRNA expression in the colon tissues was determined by northern blot analysis. Western blot analysis and immunohistochemistry in the colon tissues were performed to evaluate the expression of TLR4 protein. The expressions of TLR4 mRNA and protein were remarkably down-regulated in colon tissues of MIF-deficient mice compared with wild-type mice and up-regulated by treatment with recombinant MIF. Immunohistochemical study revealed the presence of TLR4–positive staining in mononuclear cells in the lamina propria and intraepithelial mononuclear cells as well as weak staining in epithelial cells and crypts in colon tissues of wild-type mice. In contrast, MIF-deficient mice did not show TLR4-positive staining in the colonic mucosa. In MIF-deficient mice injected with recombinant mouse MIF (rMIF), TLR4-positive staining cells were observed in colon tissues similar to the findings in wild-type mice. Administration of dextran sulfate sodium (DSS) up-regulated the expression of TLR4 in the colons of WT mice but not in those of MIF-deficient mice. Furthermore, pretreatment with rMIF up-regulated the expression of TLR4 in response to DSS in MIF-deficient mice. Our results suggest that MIF affects the expression of TLR4 in mouse colon under both normal and colitic conditions.

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Acknowledgements

This work was partly supported by Grants-in-Aid from the Japanese Ministry of Health, Labor, and Welfare. We are grateful to R. Nagashima and S. Tone for their technical assistance.

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Authors and Affiliations

  1. Department of Gastroenterology and Hematology, Hokkaido University Graduate School of Medicine, Kita-15, Nishi-7, Kita-ku, 060-8638, Sapporo, Japan

    Tatsuya Ohkawara, Hiroshi Takeda, Kencho Miyashita & Masahiro Asaka

  2. Department of Research and Development, GeneticLab, 060-8638, Sapporo, Japan

    Morie Nishiwaki, Takashi Yoshiki & Jun Nishihira

  3. Department of Molecular Immunology, Graduate School of Medicine, Chiba University, 263-8522, Chiba, Japan

    Toshinori Nakayama

  4. Department of Medical Immunology, Graduate School of Medicine, Chiba University, 263-8522, Chiba, Japan

    Masaru Taniguchi

  5. Department of Hematology and Oncology, Hokkaido University Graduate School of Medicine, 067-8638, Sapporo, Japan

    Junji Takana & Masahiro Imamura

  6. Third Department of Internal Medicine, Toyama Medical and Pharmaceutical University, 930-0194, Toyama, Japan

    Toshiro Sugiyama

Authors
  1. Tatsuya Ohkawara
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  2. Hiroshi Takeda
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  3. Kencho Miyashita
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  5. Toshinori Nakayama
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  7. Takashi Yoshiki
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  8. Junji Takana
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  9. Masahiro Imamura
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  11. Masahiro Asaka
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  12. Jun Nishihira
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Corresponding author

Correspondence to Tatsuya Ohkawara.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00418-005-0120-y

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Ohkawara, T., Takeda, H., Miyashita, K. et al. Regulation of Toll-like receptor 4 expression in mouse colon by macrophage migration inhibitory factor. Histochem Cell Biol 125, 575–582 (2006). https://doi.org/10.1007/s00418-005-0092-y

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