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The novel protein PTPIP51 exhibits tissue- and cell-specific expression

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Abstract

The expression patterns of both mRNA and protein of the novel protein tyrosine phosphatase interacting protein 51 (PTPIP51) were studied in various organs by in situ hybridization, immunoblotting, and immunocytochemistry. The protein was found in all mammalian species investigated: guinea pig, rat, mouse, pig, and human. The presence of the protein was, however, restricted to specific organs. High levels of PTPIP51 were found in epidermis and seminiferous epithelium. The expression appears to be associated with distinct stages of differentiation. While basal cells in the epidermis and spermatogonia showed no perceptible amount of PTPIP51, keratinocytes of suprabasal layers and differentiating first-order spermatocytes up to spermatids exhibited high expression. In skeletal muscle, the presence of PTPIP51 was restricted to fibers of the fast twitch type. In surface epithelia containing ciliated cells, the protein was associated with the microtubular structures responsible for ciliary movement. Furthermore, specific structures of the central nervous system, for example, neurons of the hippocampal region, ganglion cells of the autonomic nervous system, and axons of the peripheral nervous system showed a distinct staining pattern with the antibody to PTPIP51. Our data suggest that PTPIP51 might be involved in the regulation of cellular processes associated with differentiation, movement, or cytoskeletal organization.

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Acknowledgements

We are indebted to Dr. H.J. Teschemacher (Department of Pharmacology, Giessen, Germany), Dr. N.E. Fusenig, and Dr. D. Breitkreutz (DKFZ, Heidelberg, Germany) for the gift of human keratinocytes, and to Martin Bodenbenner (Giessen) and Ulrike Schlapp (Bad Nauheim) for help with cell cultures and technical assistance. We thank Dr. R.L. Snipes for linguistic revision.

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Authors and Affiliations

  1. Justus-Liebig-University Giessen, Institute of Anatomy and Cell Biology, Aulweg 123, 35385, Giessen, Germany

    Albrecht Stenzinger, Tobias Kajosch, Claudia Tag & Monika Wimmer

  2. Department of Urology and Pediatric Urology, Justus-Liebig-University Giessen, 35385, Giessen, Germany

    Klaus Steger

  3. Department of Biology, University of Konstanz, 78547, Konstanz, Germany

    Alexandra Porsche, Inka Welte & Hans Werner Hofer

Authors
  1. Albrecht Stenzinger
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  2. Tobias Kajosch
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  3. Claudia Tag
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  4. Alexandra Porsche
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  5. Inka Welte
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  6. Hans Werner Hofer
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  7. Klaus Steger
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  8. Monika Wimmer
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Correspondence to Albrecht Stenzinger.

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Tobias Kajosch died on August 9th 2004

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Stenzinger, A., Kajosch, T., Tag, C. et al. The novel protein PTPIP51 exhibits tissue- and cell-specific expression. Histochem Cell Biol 123, 19–28 (2005). https://doi.org/10.1007/s00418-004-0732-7

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  • DOI: https://doi.org/10.1007/s00418-004-0732-7

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