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Immunocytochemical localisation of calreticulin in Trypanosoma cruzi

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Abstract

Calreticulin, a Ca2+ chaperone, is found in many different locations in various eukaryotic cells, including lumen of the endoplasmic reticulum, the cell surface, perinuclear areas and cytosolic granules. In the present study, a polyclonal antibody against calreticulin was used for the immunocytochemical localisation of the protein in Trypanosoma cruzi. Labelling was observed in the endoplasmic reticulum, Golgi complex, reservosomes, flagellar pocket, cell surface, cytosol, nucleus and kinetoplast. Significant differences in labelling were observed among the three evolutive forms of the protozoan. The functional role of calreticulin in T. cruzi is discussed.

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Acknowledgements

The authors thank Dr. J.J. Cazzulo and A.J. Parodi for a gift of anti-calreticulin antibody and Antonio Bôsco Carlos for technical assistance. Work reported herein received financial support from the Conselho Nacional do Desenvolvimento Científico e Tecnológico (CNPq), Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) e Programa de Núcleos de Excelência (PRONEX), Fundação Universitária José Bonifácio (FUJB).

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Authors and Affiliations

  1. Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Centro de Ciências da Saúde, Bloco G, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, CEP 21949-900, Rio de Janeiro, Brazil

    Wanderley de Souza

  2. Laboratório de Biologia Celular e Ultraestrutura, Instituto de Microbiologia Centro de Ciências da Saúde, Bloco I, Universidade Federal do Rio de Janeiro, Cidade Universitária, Ilha do Fundão, CEP 21949-590, Rio de Janeiro, Brazil

    Thaïs Souto-Padrón

  3. Fundación Instituto Leloir, Av. Patricias Argentinas 435, C1405BEW, Buenos Aires, Argentina

    Carlos A. Labriola

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  1. Thaïs Souto-Padrón
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  2. Carlos A. Labriola
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  3. Wanderley de Souza
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Correspondence to Wanderley de Souza.

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Souto-Padrón, T., Labriola, C.A. & de Souza, W. Immunocytochemical localisation of calreticulin in Trypanosoma cruzi. Histochem Cell Biol 122, 563–569 (2004). https://doi.org/10.1007/s00418-004-0724-7

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