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Expression of P2Y1 receptors in rat taste buds

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Abstract

Extracellular nucleotides such as ATP are the signaling molecules which bind to membrane receptors (P2X ligand-gated ion channels and G-protein-coupled P2Y families). In the gustatory system, it is known that P2X receptors are expressed exclusively in nerve fibers innervating the taste buds. Also, P2Y receptors are suggested to play some important roles in taste signal transductions on the basis of the physiological studies. In the present study, we examined the expression patterns of P2Y1 receptor subtype by using reverse transcript polymerase chain reaction (RT-PCR), in situ hybridization, and immunohistochemistry. RT-PCR analyses showed that P2Y1 receptor mRNAs appeared in circumvallate papillae. P2Y1 receptor mRNA was detected in a subset of taste bud cells by in situ hybridization. By immunohistochemical analyses, P2Y1 receptor was detected in a subset of taste bud cells of fungiform, foliate, and circumvallate papillae. We showed that ATP induced a biphasic intracellular Ca2+ increase in taste cells by a Ca2+ imaging method. Furthermore, we showed by double-immunolabeling methods that P2Y1-expressing cells coexpressed both IP3R3 and SNAP-25. These results suggest that ATP may activate P2Y receptors resulting in Ca2+ release from internal stores via IP3R3. Since many SNAP-25-immunoreactive taste bud cells coexpressed P2Y1 immunoreactivity, it is suggested that P2Y1-expressing cells may possess synapses with afferent nerve fibers. The results of the present study suggest that P2Y1 receptor may play some roles in ATP-mediated signal transductions between taste bud cells and afferent taste fibers.

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Acknowledgements

This study was supported by the Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research number 14571740 to K.T. and NIH/NIDCD grants DC 05140 to T.O. and P30DC04657 to Diego Restrepo of the University of Colorado Health Science Center.

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Authors and Affiliations

  1. Department of Oral Anatomy and Neurobiology, Kyushu Dental College, Kokurakita-ku, Kitakyushu 803-8580, Japan

    Shinji Kataoka, Takashi Toyono, Y. Seta & Kuniaki Toyoshima

  2. Department of Anatomy and Neurobiology, Colorado State University, Fort Collins, Colorado, 80523, USA

    Tatsuya Ogura

  3. Department of Cellular and Developmental Biology, University of Colorado Health Science Center, Denver, Colorado, 80262, USA

    Tatsuya Ogura

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  1. Shinji Kataoka
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  2. Takashi Toyono
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  3. Y. Seta
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  4. Tatsuya Ogura
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  5. Kuniaki Toyoshima
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Correspondence to Kuniaki Toyoshima.

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Kataoka, S., Toyono, T., Seta, Y. et al. Expression of P2Y1 receptors in rat taste buds. Histochem Cell Biol 121, 419–426 (2004). https://doi.org/10.1007/s00418-004-0647-3

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