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Immunohistochemical localization of sodium–potassium ATPase in human normal stomach and gastric adenocarcinomas

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Abstract

We studied the expression pattern of Na, K-ATPase β1 subunit in human normal stomachs and in gastric adenocarcinomas by using anti-Na, K-ATPase β1 subunit-specific monoclonal antibody. Tissue samples were processed in formalin solution or in a cold acetic acid–ethanol solution, routinely processed, embedded in paraffin, and an immunoperoxidase method for Na, K-ATPase β1 subunit was performed. After antigen retrieval using a steamer in citrate buffer (pH 6.0), tissue sections initially fixed in cold acetic acid–ethanol showed intense immunoreactivity with the antibody at the lateral or basolateral cytoplasmic membrane of normal gastric epithelial cells, at the cytoplasmic membrane of gastric carcinoma cells according to the level of differentiation, and at the cytoplasmic membrane and in the cytoplasm of Schwann cells and neurons in the mesenteric plexus of the gastric wall. Acetic acid–ethanol and paraffin embedding is a useful method for the investigation of the immunohistochemical localization of Na, K-ATPase in normal and diseased tissues.

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Fig. 1A–C. Fig. 2A–C. Fig. 3.
Fig. 4A–C. Fig. 5A, B.
Fig. 6.

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Acknowledgements

We thank Prof. David Y. Graham, Houston, Tex., and Dr. Hirotoshi Tobioka, Second Department of Pathology, Sapporo Medical School, for their helpful comments and encouragement.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Sciences, School of Health Sciences, School of Medicine, Shinshu University, 3-1-1 Asahi, Matsumoto, 390-8621, Nagano, Japan

    Fumiko Terasawa, Nobuo Okumura & Hiroyoshi Ota

  2. First Department of Surgery, School of Medicine, Shinshu University, Matsumoto, 390-8621, Nagano, Japan

    Chujiro Kobayashi & Atsushi Sugiyama

  3. Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, 390-8621, Nagano, Japan

    Masayoshi Hayama & Oi Harada

Authors
  1. Chujiro Kobayashi
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  2. Masayoshi Hayama
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  3. Oi Harada
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  4. Fumiko Terasawa
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  5. Nobuo Okumura
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  6. Atsushi Sugiyama
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  7. Hiroyoshi Ota
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Correspondence to Hiroyoshi Ota.

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Kobayashi, C., Hayama, M., Harada, O. et al. Immunohistochemical localization of sodium–potassium ATPase in human normal stomach and gastric adenocarcinomas. Histochem Cell Biol 119, 317–322 (2003). https://doi.org/10.1007/s00418-003-0514-7

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  • DOI: https://doi.org/10.1007/s00418-003-0514-7

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