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Colocalisation of the protein tyrosine phosphatases PTP-SL and PTPBR7 with β4-adaptin in neuronal cells

Histochemistry and Cell Biology volume 119, pages 1–13 (2003)Cite this article

Abstract

The mouse gene Ptprr encodes the neuronal protein tyrosine phosphatases PTP-SL and PTPBR7. These proteins differ in their N-terminal domains, with PTP-SL being a cytosolic, membrane-associated phosphatase and PTPBR7 a type I transmembrane protein. In this study, we further explored the nature of the PTP-SL-associated vesicles in neuronal cells using a panel of organelle markers and noted a comparable subcellular distribution for PTP-SL and the β4-adaptin subunit of the AP4 complex. PTP-SL, PTPBR7 and β4-adaptin are localised at the Golgi apparatus and at vesicles throughout the cytoplasm. Immunohistochemical analysis demonstrated that PTP-SL, PTPBR7 and β4-adaptin are all endogenously expressed in brain. Interestingly, coexpression of PTP-SL and β4-adaptin leads to an altered subcellular localisation for PTP-SL. Instead of the Golgi and vesicle-type staining pattern, still observable for β4-adaptin, PTP-SL is now distributed throughout the cytoplasm. Although β4-adaptin was found to interact with the phosphatase domain of PTP-SL and PTPBR7 in the yeast two-hybrid system, it failed to do so in transfected neuronal cells. Our data suggest that the tyrosine phosphatases PTP-SL and PTPBR7 may be involved in the formation and transport of AP4-coated vesicles or in the dephosphorylation of their transmembrane cargo molecules at or near the Golgi apparatus.

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Acknowledgements

We thank Huib Croes and Rinske van de Vorstenbosch for excellent technical assistance, Bas Wanschers for help with the double-labelling experiments and Ineke van der Zee for help and advice. We also thank Jean Gruenberg for providing the anti-LBPA antibody, Hans Aerts for the anti-β glucocerebrosidase antibody and Mary Robinson for human β3B-adaptin cDNA. This work was supported in part by a grant from the Dutch Cancer Society to W.H.

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  1. Department of Cell Biology, Nijmegen Center for Molecular Life Sciences, University Medical Center St. Radboud, Geert Grooteplein Zuid 28, 6525 GA, Nijmegen, The Netherlands

    Gönül Dilaver, Jan Schepens, Arn van den Maagdenberg, Mietske Wijers, Barry Pepers, Jack Fransen & Wiljan Hendriks

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  1. Gönül Dilaver
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  2. Jan Schepens
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Correspondence to Wiljan Hendriks.

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Dilaver, G., Schepens, J., van den Maagdenberg, A. et al. Colocalisation of the protein tyrosine phosphatases PTP-SL and PTPBR7 with β4-adaptin in neuronal cells. Histochem Cell Biol 119, 1–13 (2003). https://doi.org/10.1007/s00418-002-0489-9

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Keywords

  • Endocytotic vesicles
  • Tissue distribution
  • Protein isoforms
  • Signal transduction
  • Subcellular localisation