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Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human

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Abstract

Purpose

To investigate the differentially expressed proteins (DEP) between high myopia and moderate myopia on the anterior corneal stroma.

Methods

Tandem mass tag (TMT) quantitative proteomics was utilized to reveal proteins. DEPs were screened by the multiple change of more than 1.2 times or less than 0.83 and the P value < 0.05. The DEPs were functional annotated by Gene Ontology (GO) terms. Proteins and protein interaction (PPI) networks were conducted with String online tool. Parallel reaction monitoring (PRM) data processing was used to verify the TMT proteomics results.

Results

There are 36 DEPs between high myopia and moderate myopia on the anterior corneal stroma, of which 11 proteins are upregulated, 25 proteins are downregulated. The GO analysis demonstrated keratinocyte migration and structural constituent of cytoskeleton that are significantly changed with most of the proteins decreased in high myopic corneas. Keratin 16 (KRT16) and erythrocyte membrane protein band 4.1-like protein 4B are the only two proteins involved in both functions. The PPI analysis showed keratin type II cytoskeletal 6A (KRT6A) and KRT16 that have strong connections. Immunoglobulin lambda variable 8–61(IGLV8-61) and nicotinamide phosphoribosyl transferase (NAMPT) have consistent results with the TMT.

Conclusions

The high myopic corneas have 36 DEPs compared to the moderate myopic corneas on the anterior corneal stroma. Keratinocyte migrations and structural constituent of cytoskeleton are weakened in high myopic corneas, which may partly account for the lower corneal biomechanics in high myopic eyes. The lower expressed KRT16 plays important roles in high myopic corneas.

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Abbreviations

BP:

Biological processes

CC:

Cellular components

DEP:

Differentially expressed proteins

GO:

Gene Ontology

KEGG:

The Kyoto Encyclopedia of Genes and Genomes analysis

KRT:

Keratin

MF:

Molecular function

PPI:

Protein-protein interactions

PRM:

Parallel reaction monitoring

SMILE:

Femtosecond laser small incision lenticule extraction

TMT:

Tandem mass tag

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Acknowledgements

Thanks for Tianjin Eye Hospital for providing the study subjects.

Funding

This study was funded by the National Natural Science Foundation of China (Grant No. 81900828, Wenjing Wu) and the Beijing Municipal Commission of Science and Technology Capital Clinical Diagnosis and Treatment Technology Research and Transformation Application (Grant Number Z201100005520043, Fengju Zhang).

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Authors and Affiliations

  1. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, No. 1, Dongjiaomin Lane, Dongcheng District, Beijing, 100730, China

    Wenjing Wu, Yushan Xu & Fengju Zhang

Authors
  1. Wenjing Wu
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  2. Yushan Xu
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Contributions

Wenjing Wu: study design; data collection, analysis and interpretation, and writing and revising the manuscript. Yushan Xu: revising the manuscript. Fengju Zhang: funding collection, study design, data analysis, and revising the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Fengju Zhang.

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Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the Institutional Review Board (IRB) of Tianjin Eye Hospital and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study. This article does not contain any studies with animals performed by any of the authors.

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The authors declare no competing interests.

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Wu, W., Xu, Y. & Zhang, F. Comparisons of the protein expressions between high myopia and moderate myopia on the anterior corneal stroma in human. Graefes Arch Clin Exp Ophthalmol 261, 3549–3558 (2023). https://doi.org/10.1007/s00417-023-06158-2

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  • DOI: https://doi.org/10.1007/s00417-023-06158-2

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