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FOXM1 promotes TGF-β2-induced injury of human lens epithelial cells by up regulating VEGFA expression

  • Basic Science
  • Published:
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Abstract

Objective

To explore whether Fork head box protein M1 (FOXM1) is involved in TGF-β2-induced injury of human lens epithelial cells and its related mechanism.

Methods

Human lens epithelium samples from cataract patients and healthy controls were collected. A cellular epithelial injury model was established by treating HLE-B3 cells with TGF-β2. QPCR, immunoblot assays were performed to detect the levels of FOXM1 in human cataract samples and the lens epithelial injury cell model. FOXM1 siRNA and pcDNA3.1-FOXM1 plasmids were transfected into the cells to knockdown and overexpress FOXM1, respectively. MTT and wound closure and transwell assays were performed to analyze cell proliferation and migration in HLE-B3 cells. Immunoblot assays were also conducted to detect the effects of FOXM1 on EMT, VEGFA and MAPK/ERK signaling.

Results

We found high expression of FOXM1 in lens tissues of cataract patients. Silencing of FOXM1 in TGF-β2-induced HLE-B3 cells suppressed cell proliferation, migration, and the EMT process. Mechanistically, we found that downregulation of FOXM1 inhibited the VEGFA/MAPK signaling pathway in TGF-β2-induced HLE-B3 cells.

Conclusion

FOXM1 promoted TGF-β2-induced injury of human lens epithelial cells (hLECs) by promoting VEGFA expression. FOXM1 could be a potential drug target for the treatment of ocular diseases.

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Data availability

All data are included in this published article.

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Authors and Affiliations

  1. Department of Ophthalmology, Kashgar Prefecture Second People’s Hospital, Kashgar, 844000, Xinjiang, China

    Xuemei Li & Wei Gao

  2. Department of Ophthalmology, The First Affiliated Hospital of Chongqing Medical University, Chongqing Key Lab of Ophthalmology, Chongqing Eye Institute, Chongqing Branch of National Clinical Research Center for Ocular Diseases, No. 1 Youyi Road, Yuzhong District, Chongqing, China

    Yanlai Zhang

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Contributions

All authors contributed to the study conception and design. Material preparation and the experiments were performed by Xuemei Li. Data collection and analysis were performed by Wei Gao. The first draft of the manuscript was written by Yanlai Zhang and all authors commented on previous versions of the manuscript.

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Correspondence to Yanlai Zhang.

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Ethical approval was obtained from the Ethics Committee of Kashgar Second People's Hospital of Xinjiang Uygur Autonomous Region.

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Li, X., Gao, W. & Zhang, Y. FOXM1 promotes TGF-β2-induced injury of human lens epithelial cells by up regulating VEGFA expression. Graefes Arch Clin Exp Ophthalmol 261, 2547–2555 (2023). https://doi.org/10.1007/s00417-023-06065-6

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  • DOI: https://doi.org/10.1007/s00417-023-06065-6

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