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Tear cytokines as potential biomarkers in non-infectious uveitis: post hoc analysis of a randomised clinical trial

Abstract

Purpose

The study aimed to evaluate cytokines in tears as potential biomarkers in non-infectious uveitis.

Methods

Tear samples were obtained using Schirmer strips from 50 patients enrolled in a randomised controlled study of simvastatin in non-infectious uveitis and from a control group of 30 healthy participants. The concentrations of IL-6, IL-8, IL-10, and IFN-γ in tears were measured at the study’s baseline and again after 8 weeks of treatment using commercial enzyme-linked immunosorbent assay kits.

Results

The concentrations of tears IL-6 and IL-10 were increased in patients with non-infectious uveitis in comparison with the healthy participants. Longer disease duration was associated with elevated levels of IL-6. The concentrations of the studied cytokines in tears did not correlate with the extent of eye inflammation at baseline. No link between the changes in cytokine levels and changes in clinical parameters during treatment was observed. Baseline IL-10 concentrations independently predicted the development of the clinical response to treatment at weeks 4 and 8.

Conclusion

Although elevated in non-infectious uveitis patients, tear cytokine levels do not correlate with eye inflammation and are not sensitive to change after treatment. However, the level of IL-10 may be a predictive biomarker of response to the treatment of uveitis.

Trial registration

NCT04183387

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Authors and Affiliations

Authors

Contributions

All authors contributed to the study conception and design. Material preparation and data collection were performed by Anastasia Biruukova and Natalia Kalinovskaya; analyses were performed by Ivan Shirinsky and Valery Shirinsky. The first draft of the manuscript was written by Ivan Shirinsky, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ivan V. Shirinsky.

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Ethical approval

All procedures performed in this study were in accordance with the ethical standards of the LEC attached to Municipal City Hospital No. 1, Novosibirsk, and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants included in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Cite this article

Shirinsky, I.V., Biryukova, A.A., Kalinovskaya, N.Y. et al. Tear cytokines as potential biomarkers in non-infectious uveitis: post hoc analysis of a randomised clinical trial. Graefes Arch Clin Exp Ophthalmol 258, 1813–1819 (2020). https://doi.org/10.1007/s00417-020-04707-7

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  • DOI: https://doi.org/10.1007/s00417-020-04707-7

Keywords

  • Uveitis
  • Autoimmune
  • Non-infectious
  • Hydroxymethylglutaryl-CoA reductase inhibitors
  • Statins
  • Cytokines
  • Tears