Autoantibody against aldehyde dehydrogenase 2 could be a biomarker to monitor progression of Graves’ orbitopathy

  • Kai-Chun Cheng
  • Yu-Jen Wu
  • Kai-Hung Cheng
  • Kai-Yuan Cheng
  • Kuo-Jen Chen
  • Wen-Chuan Wu
  • Po-Yen Lee
  • Cheng-Hsien Chang
Oculoplastics and Orbit



This study surveyed the novel autoantigens expressed in the orbital fat tissue of patients with Graves’ orbitopathy (GO) and explored the possibility of the autoantibodies against novel autoantigens as biomarkers for GO.


We used immuno-proteomic methods to survey novel autoantigens expressed in the orbit fat tissue of GO patients and confirmed by enzyme-linked immunosorbent assay (ELISA).


One protein spot (aldehyde dehydrogenase 2 (ALDH2)) revealed high reactivity with the GO serum than did the healthy control serum and was further verified by ELISA. We found that the plasma anti-ALDH2 antibody level was increased in GO patients compared to healthy control donors. In addition, anti-ALDH2 antibody level was correlated with GO activity classified by clinical activity score(r = 0.588, p < 0.001, using Pearson’s correlation).


These increased levels of anti-ALDH2 antibody in GO serum suggested that ALDH2 could attribute target autoantigen in GO, and anti-ALDH2 autoantibody might serve as a biomarker for GO and help to predict disease activity.


Graves’ orbitopathy Autoantigen Proteomic analysis Aldehyde dehydrogenase 2 Orbital fat 



This study was supported by Grant kmhk-98-024 from Kaohsiung Municipal Hsiao-Kang Hospital. The sponsor had no role in the design or conduct of this research.

Compliance with ethical standards

Conflict of interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Kai-Chun Cheng
    • 1
    • 2
    • 3
  • Yu-Jen Wu
    • 4
  • Kai-Hung Cheng
    • 5
    • 6
  • Kai-Yuan Cheng
    • 7
  • Kuo-Jen Chen
    • 1
  • Wen-Chuan Wu
    • 2
    • 8
  • Po-Yen Lee
    • 2
  • Cheng-Hsien Chang
    • 2
    • 8
  1. 1.Department of OphthalmologyKaohsiung Municipal Hsiao-kang HospitalKaohsiungTaiwan
  2. 2.Department of OphthalmologyKaohsiung Medical University HospitalKaohsiungTaiwan
  3. 3.Department of OptometryShu-Zen junior College of Medicine and ManagementKaohsiungTaiwan
  4. 4.Department of Biological Science and TechnologyMeiho UniversityPingtungTaiwan
  5. 5.Division of Cardiology, Department of Internal MedicineKaohsiung Medical University HospitalKaohsiungTaiwan
  6. 6.Department of Internal Medicine, Faculty of Medicine, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan
  7. 7.Department of Otolaryngology, Head and Neck SurgeryMinistry of Health and Welfare Pingtung HospitalPingtungTaiwan
  8. 8.Department of Ophthalmology, Faculty of Medicine, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan

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