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Inter-method agreement in retinal blood vessels diameter analysis between Dynamic Vessel Analyzer and optical coherence tomography

  • Lucia Benatti
  • Federico Corvi
  • Livia Tomasso
  • Stefano Mercuri
  • Lea Querques
  • Fulvio Ricceri
  • Francesco Bandello
  • Giuseppe QuerquesEmail author
Retinal Disorders

Abstract

Purpose

To analyze the inter-methods agreement in arteriovenous ratio (AVR) evaluation between spectral-domain optical coherence tomography (SD-OCT) and Dynamic Vessel Analyzer (DVA).

Methods

Healthy volunteers underwent DVA and SD-OCT examination. AVR was measured by SD-OCT using the four external lines of the optic nerve head-centered 7-line cube and by DVA using an automated AVR estimation. The mean AVR was calculated, twice, separately by two independent readers for each tool.

Results

Twenty-two eyes of 11 healthy subjects (five women and six men, mean age 35) were included. AVR analysis by DVA showed high inter-observer agreement between reader 1 and 2, and high intra-observer agreement for both reader 1 and reader 2. With regard to AVR analysis on SD-OCT, we found high inter-observer agreement between reader 1 and 2, and low intra-observer agreement for reader 2 but high intra-observer agreement for reader 1. Overall, the mean AVR measured on SD-OCT turned out to be significantly higher than mean AVR measured through DVA (reader 1, 0.9023 ± 0.06 vs 0.8036 ± 0.08; p < 0.001, and reader 2, 0.9067 ± 0.06 vs 0.8083 ± 0.05; p= 0.003).

Conclusions

No inter-method agreement in AVR could be detected in the present study due to bias in measurements (shift between DVA and SD-OCT). We found significant difference in the two noninvasive methods for AVR measurement, with a tendency for SD-OCT to overestimate retinal vascular caliber in comparison to DVA. This may be useful for achieving greater accuracy in the evaluation of retinal vessel in ocular as well as systemic diseases.

Keywords

Arteriovenous ratio Dynamic vessel analyzer Inter-methods agreement Reproducibility Semi-automated analysis Spectral-domain optical coherence tomography 

Notes

Compliance with ethical standards

The authors have no proprietary, funding, or conflicts of interest to disclose.

Informed consent was obtained from all subjects in agreement with the Declaration of Helsinki for research involving human subjects.

No animals were involved in the research.

Funding

No funding was received for this research.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflict of interest

All authors certify that they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers’ bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge, or beliefs) in the subject matter or materials discussed in this manuscript.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  • Lucia Benatti
    • 1
  • Federico Corvi
    • 1
  • Livia Tomasso
    • 1
  • Stefano Mercuri
    • 1
  • Lea Querques
    • 1
  • Fulvio Ricceri
    • 1
  • Francesco Bandello
    • 1
  • Giuseppe Querques
    • 1
    Email author
  1. 1.Department of OphthalmologyUniversity Vita-Salute, IRCCS Ospedale San RaffaeleMilanItaly

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