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Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron microscopic study

  • Retinal Disorders
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

To describe characteristics of epiretinal cells at the vitreoretinal interface by correlative light and electron microscopy (CLEM).

Methods

Epiretinal membrane (ERM) specimens and internal limiting membrane (ILM) specimens were harvested by sequential peeling during vitrectomy from 27 eyes with idiopathic epiretinal gliosis, and processed for CLEM. Intraoperatively, the presence of posterior vitreous detachment (PVD) was documented. We used anti-vimentin, anti-α-smooth muscle actin (α-SMA), and anti-CD45 as primary antibodies. A fluorescein-tagged immunonanogold cluster was used as secondary antibody and visualized under the fluorescence and transmission electron microscope.

Results

We demonstrated CD45-positive cells specifically labelled at their plasma membranes with ultrastructural features known for hyalocytes, such as oval nucleus with marginal chromatin, vacuoles, dense granules, and thin cytoplasmic protrusions. CD45-positive cells were mostly located on a thick layer of native vitreous collagen. They were covered by newly formed collagen strands with multilayered proliferation of myofibroblasts. We also demonstrated immunoreactivity for vimentin and alpha-SMA. Cell fragments with positive labelling for α-SMA and vimentin were not only found on the vitreal side of the ILM, but also on the retinal side.

Conclusions

By CLEM, the majority of CD45-positive cells in epiretinal cell proliferation were characterized as hyalocytes. In the context of anomalous PVD and vitreoschisis, ultrastructural features and topographic localization of hyalocytes suggest that these cells play a significant role in ERM formation. CLEM enables a more accurate characterization of epiretinal cell proliferation, and therefore, contributes to a better understanding of the pathogenesis of diseases at the vitreoretinal interface.

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Financial support

This study was supported by the Ludwig-Maximilians-University Grant for Research and Education (FöFoLe, ID # 822).

Conflict of interest

The authors have no proprietary interest in any respect of this study.

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany

    Ricarda G. Schumann, Arnd Gandorfer, Jean Ziada, Renate Scheler, Markus M. Schaumberger, Armin Wolf, Anselm Kampik & Christos Haritoglou

  2. Vitreoretinal Pathology and Electron Microscopy, Department of Ophthalmology, Ludwig-Maximilians-University, Mathildenstrasse 6, 80336, Munich, Germany

    Ricarda G. Schumann

Authors
  1. Ricarda G. Schumann
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  2. Arnd Gandorfer
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  3. Jean Ziada
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  4. Renate Scheler
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  5. Markus M. Schaumberger
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  6. Armin Wolf
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  7. Anselm Kampik
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  8. Christos Haritoglou
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Correspondence to Ricarda G. Schumann.

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Schumann, R.G., Gandorfer, A., Ziada, J. et al. Hyalocytes in idiopathic epiretinal membranes: a correlative light and electron microscopic study. Graefes Arch Clin Exp Ophthalmol 252, 1887–1894 (2014). https://doi.org/10.1007/s00417-014-2841-x

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  • DOI: https://doi.org/10.1007/s00417-014-2841-x

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