Skip to main content

Advertisement

SpringerLink
Log in

Level of vitreous alpha-B crystallin in eyes with rhegmatogenous retinal detachment

  • Retinal Disorders
  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

Purpose

Alpha-B crystallin (αB-crystallin) is a molecular chaperone, and one of the small heat shock proteins (sHSPs). αB-crystallin has been shown to have neuroprotective properties. The purpose of this study was to determine the level of αB-crystallin in eyes with a rhegmatogenous retinal detachment (RRD).

Methods

Vitreous samples were collected before vitrectomy from 32 eyes of 32 consecutive patients with RRD. The concentration of αB-crystallin was measured by enzyme-linked immunosorbent assay (ELISA). Vitreous samples were also collected before vitrectomy from 16 patients with an idiopathic macular hole and epiretinal membrane, and used as controls.

Results

The mean age was 56 years (range, 31–67 years) in the RRD cases and 66 years (range, 49–77 years) in the controls. The mean level of αB-crystallin was significantly higher in the RRD cases (36.7 ± 37.4 ng/ml) than that in controls (8.5 ± 12.2 ng/ml; P = 0.0028).

Conclusions

The increased level of αB-crystallin suggests that αB-crystallin may play a neuroprotective role by lessening retinal cell death after a retinal detachment.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Kannan R, Sreekumar PG, Hinton DR (2012) Novel roles for alpha-crystallins in retinal function and disease. Prog Retin Eye Res 31:576–604. doi:10.1016/j.preteyeres.2012.06.001

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Lowe J, McDermott H, Pike I, Spendlove I, Landon M, Mayer RJ (1992) alpha B crystallin expression in non-lenticular tissues and selective presence in ubiquitinated inclusion bodies in human disease. J Pathol 166:61–68. doi:10.1002/path.1711660110

    Article  CAS  PubMed  Google Scholar 

  3. Pineda R 2nd, Chan CC, Ni M, Hayden BJ, Johnson MA, Nickerson J, Chader GJ (1993) Human retinoblastoma cells express alpha B-crystallin in vivo and in vitro. Curr Eye Res 12:239–245

    Article  PubMed  Google Scholar 

  4. Launay N, Tarze A, Vicart P, Lilienbaum A (2010) Serine 59 phosphorylation of {alpha}B-crystallin down-regulates its anti-apoptotic function by binding and sequestering Bcl-2 in breast cancer cells. J Biol Chem 285:37324–37332. doi:10.1074/jbc.M110.124388

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Goplen D, Bougnaud S, Rajcevic U, Boe SO, Skaftnesmo KO, Voges J, Enger PO, Wang J, Tysnes BB, Laerum OD, Niclou S, Bjerkvig R (2010) alphaB-crystallin is elevated in highly infiltrative apoptosis-resistant glioblastoma cells. Am J Pathol 177:1618–1628. doi:10.2353/ajpath.2010.090063

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Nakata K, Crabb JW, Hollyfield JG (2005) Crystallin distribution in Bruch’s membrane–choroid complex from AMD and age-matched donor eyes. Exp Eye Res 80:821–826. doi:10.1016/j.exer.2004.12.011

    Article  CAS  PubMed  Google Scholar 

  7. Dong Z, Kase S, Ando R, Fukuhara J, Saito W, Kanda A, Murata M, Noda K, Ishida S (2012) Alphab-crystallin expression in epiretinal membrane of human proliferative diabetic retinopathy. Retina 32:1190–1196. doi:10.1097/IAE.0b013e318233ab9c

    Article  CAS  PubMed  Google Scholar 

  8. Hu WF, Gong L, Cao Z, Ma H, Ji W, Deng M, Liu M, Hu XH, Chen P, Yan Q, Chen HG, Liu J, Sun S, Zhang L, Liu JP, Wawrousek E, Li DW (2012) alphaA- and alphaB-crystallins interact with caspase-3 and Bax to guard mouse lens development. Curr Mol Med 12:177–187

    Article  CAS  PubMed  Google Scholar 

  9. Mao YW, Liu JP, Xiang H, Li DW (2004) Human alphaA- and alphaB-crystallins bind to Bax and Bcl-X(S) to sequester their translocation during staurosporine-induced apoptosis. Cell Death Differ 11:512–526. doi:10.1038/sj.cdd.4401384

    Article  CAS  PubMed  Google Scholar 

  10. Adhikari AS, Singh BN, Rao KS, Rao Ch M (2011) alphaB-crystallin, a small heat shock protein, modulates NF-kappaB activity in a phosphorylation-dependent manner and protects muscle myoblasts from TNF-alpha induced cytotoxicity. Biochim Biophys Acta 1813:1532–1542. doi:10.1016/j.bbamcr.2011.04.009

    Article  CAS  PubMed  Google Scholar 

  11. Mao YW, Xiang H, Wang J, Korsmeyer S, Reddan J, Li DW (2001) Human bcl-2 gene attenuates the ability of rabbit lens epithelial cells against H2O2-induced apoptosis through down-regulation of the alpha B-crystallin gene. J Biol Chem 276:43435–43445. doi:10.1074/jbc.M102195200

    Article  CAS  PubMed  Google Scholar 

  12. Kamradt MC, Chen F, Cryns VL (2001) The small heat shock protein alpha B-crystallin negatively regulates cytochrome c- and caspase-8-dependent activation of caspase-3 by inhibiting its autoproteolytic maturation. J Biol Chem 276:16059–16063. doi:10.1074/jbc.C100107200

    Article  CAS  PubMed  Google Scholar 

  13. Yaung J, Jin M, Barron E, Spee C, Wawrousek EF, Kannan R, Hinton DR (2007) alpha-Crystallin distribution in retinal pigment epithelium and effect of gene knockouts on sensitivity to oxidative stress. Mol Vis 13:566–577

    CAS  PubMed Central  PubMed  Google Scholar 

  14. Vazquez-Chona F, Song BK, Geisert EE Jr (2004) Temporal changes in gene expression after injury in the rat retina. Invest Ophthalmol Vis Sci 45:2737–2746. doi:10.1167/iovs.03-1047

    Article  PubMed Central  PubMed  Google Scholar 

  15. Liu S, Li J, Tao Y, Xiao X (2007) Small heat shock protein alphaB-crystallin binds to p53 to sequester its translocation to mitochondria during hydrogen peroxide-induced apoptosis. Biochem Biophys Res Commun 354:109–114. doi:10.1016/j.bbrc.2006.12.152

    Article  CAS  PubMed  Google Scholar 

  16. Sreekumar PG, Spee C, Ryan SJ, Cole SP, Kannan R, Hinton DR (2012) Mechanism of RPE cell death in alpha-crystallin deficient mice: a novel and critical role for MRP1-mediated GSH efflux. PLoS ONE 7:e33420. doi:10.1371/journal.pone.0033420

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  17. Machemer R (1968) Experimental retinal detachment in the owl monkey. II. Histology of retina and pigment epithelium. Am J Ophthalmol 66:396–410

    Article  CAS  PubMed  Google Scholar 

  18. Anderson DH, Stern WH, Fisher SK, Erickson PA, Borgula GA (1981) The onset of pigment epithelial proliferation after retinal detachment. Invest Ophthalmol Vis Sci 21:10–16

    CAS  PubMed  Google Scholar 

  19. Kroll AJ, Machemer R (1968) Experimental retinal detachment in the owl monkey. 3. Electron microscopy of retina and pigment epithelium. Am J Ophthalmol 66:410–427

    Article  CAS  PubMed  Google Scholar 

  20. Cook B, Lewis GP, Fisher SK, Adler R (1995) Apoptotic photoreceptor degeneration in experimental retinal detachment. Invest Ophthalmol Vis Sci 36:990–996

    CAS  PubMed  Google Scholar 

  21. Arroyo JG, Yang L, Bula D, Chen DF (2005) Photoreceptor apoptosis in human retinal detachment. Am J Ophthalmol 139:605–610. doi:10.1016/j.ajo.2004.11.046

    Article  PubMed  Google Scholar 

  22. Hisatomi T, Sakamoto T, Murata T, Yamanaka I, Oshima Y, Hata Y, Ishibashi T, Inomata H, Susin SA, Kroemer G (2001) Relocalization of apoptosis-inducing factor in photoreceptor apoptosis induced by retinal detachment in vivo. Am J Pathol 158:1271–1278. doi:10.1016/S0002-9440(10)64078-3

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  23. Zacks DN, Hanninen V, Pantcheva M, Ezra E, Grosskreutz C, Miller JW (2003) Caspase activation in an experimental model of retinal detachment. Invest Ophthalmol Vis Sci 44:1262–1267

    Article  PubMed  Google Scholar 

  24. Zacks DN, Zheng QD, Han Y, Bakhru R, Miller JW (2004) FAS-mediated apoptosis and its relation to intrinsic pathway activation in an experimental model of retinal detachment. Invest Ophthalmol Vis Sci 45:4563–4569. doi:10.1167/iovs.04-0598

    Article  PubMed  Google Scholar 

  25. Trichonas G, Murakami Y, Thanos A, Morizane Y, Kayama M, Debouck CM, Hisatomi T, Miller JW, Vavvas DG (2010) Receptor interacting protein kinases mediate retinal detachment-induced photoreceptor necrosis and compensate for inhibition of apoptosis. Proc Natl Acad Sci U S A 107:21695–21700. doi:10.1073/pnas.1009179107

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  26. Sreekumar PG, Kannan R, Kitamura M, Spee C, Barron E, Ryan SJ, Hinton DR (2010) alphaB crystallin is apically secreted within exosomes by polarized human retinal pigment epithelium and provides neuroprotection to adjacent cells. PLoS ONE 5:e12578. doi:10.1371/journal.pone.0012578

    Article  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by Japan Society for the Promotion of Science KAKENHI 23791966, Grant-in-Aid for Young Scientists (B) (TB). The authors indicate no financial support or financial conflict of interest involved in design and conduct of study, collection of data, management, analysis and interpretation of data, and preparation, review, or approval of manuscript. The authors thank Professor Duco Hamasaki of the Bascom Palmer Eye Institute of the University of Miami for his critical discussion and editing the final manuscript.

Author information

Authors and Affiliations

  1. Department of Ophthalmology and Visual Science, Chiba University Graduate School of Medicine, 1-8-1 Inohana, Chuo-ku, Chiba, 260-0856, Japan

    Takayuki Baba, Toshiyuki Oshitari & Shuichi Yamamoto

Authors
  1. Takayuki Baba
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Toshiyuki Oshitari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shuichi Yamamoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Takayuki Baba.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Baba, T., Oshitari, T. & Yamamoto, S. Level of vitreous alpha-B crystallin in eyes with rhegmatogenous retinal detachment. Graefes Arch Clin Exp Ophthalmol 253, 1251–1254 (2015). https://doi.org/10.1007/s00417-014-2815-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00417-014-2815-z

Keywords

Search

Navigation