Abstract
Background
To characterize the effects of intravitreal injections of iodoacetic acid (IAA) in comparison to its systemic application as a measure to induce unilateral photoreceptor degeneration.
Methods
Seven-week-old C57BL/6 J mice received either intravitreal injections of IAA or systemic treatment (intraperitoneal vs intravenous) and were observed in the following 5 weeks using ERG, OCT, and histology.
Results
Systemic treatment with IAA induced high toxic effects and a high mortality in contrast to the intravitreal injection. Intraperitoneal application had no effect on the retina. Intravenous application of 2 × 30 mg/kg BW IAA (time between injections 3.5 h) resulted in an extinction of the ERG and a thinning of the retina, in particular of the outer nuclear layer (ONL) indicating photoreceptor degeneration. Animals receiving intravitreal injections developed cataracts already at low concentrations (up to 100 % at 0.25 mg/kg BW). Higher intravitreal IAA doses led to extinguished ERGs. In histology, a thinning of the entire retina was observed that was most prominent in the inner part of the retina.
Conclusions
In contrast to intraperitoneal administration, intravenous application of IAA led to a selective photoreceptor degeneration. After intravitreal injection, dense cataracts were already observed at concentrations lower than those needed to induce changes in the ERG. ERG results must be interpreted carefully. A thinning of all retinal layers rather than a specific outer retinal degeneration was observed upon intravitreal injection. IAA is not a useful model to induce outer retinal degeneration in mice.
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Acknowledgments
This work was supported by the DFG grants WA 1472/6–1 to PW and MU 3036/3–1 to FM. The authors thank Christoph Aretzweiler (Institute of Complex Systems, Cellular Biophysics, ICS-4, Forschungszentrum Jülich GmbH, 52428 Jülich, Germany) for the excellent support in preparation and staining of the eyes.
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This work is part of a doctoral thesis at the University of Veterinary Medicine Hannover (December 2013).
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Rösch, S., Johnen, S., Mazinani, B. et al. The effects of iodoacetic acid on the mouse retina. Graefes Arch Clin Exp Ophthalmol 253, 25–35 (2015). https://doi.org/10.1007/s00417-014-2652-0
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DOI: https://doi.org/10.1007/s00417-014-2652-0