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Surface-modified silicone foils for intraocular implantation

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Abstract

Background

Implantation of silicone materials like iris diaphragms into the eye can be complicated by cell migration and attachment. We studied polydimethylsiloxane (PDMS) foils coated with isocyanate terminated, star-shaped poly(ethylene glycol-stat-propylene glycol) (NCO-sP(EO-stat-PO)) equipped with heparin towards the inhibition of cell attachment without influencing cell viability.

Methods

Mouse fibroblasts L929 were cultured and seeded onto sterilized pieces of either uncoated NCO-sP(EO-stat-PO) or heparin-NCO-sP(EO-stat-PO) loaded foils. Polyvinylchloride (PVC) foils served as the positive control and biomembranes as the negative control. The cultured cells were examined after 24 h for cell viability and adhesion by fluorescence microscopy; morphological cell changes were documented after hemalaun staining. Cell density was measured and quantification of cell proliferation was assessed by a BrdU test; quantification of cell activity was analyzed by a WST-1 test.

Results

The fibroblasts’ cell viability was excellent on all tested foils except the toxic PVC foil. NCO-sP(EO-stat-PO) coating provided significantly reduced cell activity. On heparin-loaded coatings, cells were viable and less dense but showed almost the same cell proliferation and cell activity as on the negative control. NCO-sP(EO-stat-PO) coated, heparin loaded foils proved high biocompatibility and reduced cell adhesion.

Conclusions

Both NCO-sP(EO-stat-PO)-coated foils with and without heparin seemed to be a viable implantation material for less cell migration, attachment, and reduced implant complications. Conclusive we give a recommendation for further studies on the intraocular implantation in particular for the NCO-sP(EO-stat-PO)-coated foils.

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Acknowledgements

The technical assistance of this research project by the Interdisciplinary Centre for Clinical research "BIOMAT" within the Faculty of Medicine at the RWTH Aachen University is gratefully appreciated.

Supported by a grant (TV-B109) from the Interdisciplinary Centre for Clinical Research "BIOMAT" within the faculty of Medicine at the RWTH Aachen University and a grant by the START program of the medical faculty of the RWTH Aachen University.

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Authors and Affiliations

  1. Department of Ophthalmology, RWTH Aachen University, Pauwelsstraße 30, 52074, Aachen, Germany

    Sarah Fischer, Dörthe Carstesen, Peter Walter & Andreas W. A. Weinberger

  2. Institute of Technical and Macromolecular Chemistry, RWTH Aachen University, Worringerweg 1, 52074, Aachen, Germany

    Doris Klee

Authors
  1. Sarah Fischer
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  2. Dörthe Carstesen
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  3. Doris Klee
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  4. Peter Walter
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  5. Andreas W. A. Weinberger
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Correspondence to Sarah Fischer.

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Fischer, S., Carstesen, D., Klee, D. et al. Surface-modified silicone foils for intraocular implantation. Graefes Arch Clin Exp Ophthalmol 250, 823–827 (2012). https://doi.org/10.1007/s00417-012-1956-1

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  • DOI: https://doi.org/10.1007/s00417-012-1956-1

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