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Mechanical behavior of scleral fibroblasts in experimental myopia

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Abstract

Background

The study aims to determine the changes in the biomechanical properties of the anterior and extreme posterior portions of experimental near-sighted eyes by examining the mechanical behavior of guinea pig scleral desmocytes, thus finding a new approach to the pathogenesis of myopia and their corresponding therapies.

Methods

Guinea pigs (2 weeks old) were numbered and assigned into three groups (A, B, and C) with ten guinea pigs each. Concave lens-induced myopic (LIM) animal models were prepared via the out-of-focus method. The other eye in the same guinea pig served as the self-control (SC) group. After modeling groups A, B, and C for 6, 15, and 30 days respectively, the lenses from the guinea pigs in the experimental group were removed. The scleral fibroblasts in each group were cultured, and passaged twice in vitro. The micropipette aspiration technique coupled with a viscoelastic solid model was utilized to investigate the viscoelastic properties of the scleral fibroblasts in normal and myopic guinea pigs. The mechanical behavior of the scleral desmocytes of the LIM and SC groups were compared.

Results

The mechanical behavior of the scleral desmocytes was compared between the LIM and SC groups. The Young's modulus at equilibrium and the apparent cellular viscosity of the anterior portion of the sclera in the LIM group at 6 days and 15 days after myopic induction were not significantly different from that of the SC group (P < 0.05). However, the results for the anterior portions of the sclera in the LIM group at 30 days were significantly higher than those of the LIM group at 6 and 15 days, as well as those in the SC group (P < 0.05). The Young's modulus at equilibrium and the apparent cellular viscosity of the extreme posterior portions of the sclera in the LIM group at 6 days after myopic induction not significantly from those of the SC group (P < 0.05). However, the results for the extreme posterior portions of the sclera in the LIM group after 15 days and 30 days were significantly higher than those in the LIM group at 6 days and the SC group (P < 0.05).

Conclusions

The Young's modulus at equilibrium or apparent cellular viscosity of all the anterior portions of the sclera in the LIM group were longer than those in the SC group at 30 days after the induction, and the results for all the extreme posterior portions of the LIM group were larger than those of the SC group on the 15th and 30th day. Therefore, the Young's modulus and apparent viscosity of the anterior and extreme posterior portions of the sclera changed on the 15th and 30th day after induction respectively.

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References

  1. Hu D (2004) Progress in the study of myopic etiology and pathogenesis. Chin J Optomet & Ophthalmol 6(1):1–5

    Google Scholar 

  2. Rada JA, Nickla DL, Troilo D (2000) Decreased proteoglycan synthesis associated with form deprivation myopia in mature primate eyes. Invest Ophthalmol Vis Sci 41( I):2050–2058

    PubMed  CAS  Google Scholar 

  3. Phillips JR, Khalaj M, McBrien NA (2000) Induced myopia associated with increased creep in chick and tree shrew eyes. Invest Ophthalmol Vis Sci 41:2028–2034

    PubMed  CAS  Google Scholar 

  4. McBrien NA, Jobling AI, Gentle A (2009) Biomechanics of the sclera in myopia: extracellular and cellular factors.Optom Vis Sci 86(1):E23−E30

    Article  PubMed  Google Scholar 

  5. Cui W, Bryant MR, Sweet PM, McDonnell PJ (2004) Changes in gene expression in response to mechanicalstrain in human scleral fibroblasts. Exp Eye Res 78:275–284

    Article  PubMed  CAS  Google Scholar 

  6. Ouyang C, Hu W, Chu R (2002) Effects of concave lens on eyes of guinea pig. Chin Ophthal Res 20(5):391–393

    Google Scholar 

  7. Sato M, Theret DP, Wheeler LT, Ohshima N, Nerem RM (1990) Application of the micropipette technique to the measurement of cultured porcine aortic endothelial cell viscoelastic properties. J Biomech Eng 112(3):263–268

    Article  PubMed  CAS  Google Scholar 

  8. Wang C, Chen W, Hao L, Wu H, Liu Y, Tan S (2003) Scleral biomechanical properties in high myopia. Chin Ophthal Res 21(2):113–115

    Google Scholar 

  9. Wang Y, Li T, Chen W (2007) The relationship of scleral collagen and its biomechanical properties. J Taiyuan Univ Technol 38(4):371–373

    Google Scholar 

  10. Sun Z, Wang C, Jin S, Wang J, Chen W (2006) A biomechanical study of the sclera in experimental myopia. Chin J Optomet & Ophthalmol 8(4):209–213

    Google Scholar 

  11. Ingber DE (2003) Cellular tensegrity I: Cell structure and hierarchical systems biology. J Cell Sci 1167:1157–1173

    Article  Google Scholar 

  12. Stamenović D (2005) Effects of cytoskeletal prestress on cell rheological behavior. Acta Biomaterialia 1(3):255–262

    Article  PubMed  Google Scholar 

  13. Wang N, Tolia N, rrelykke IM, Chen J, Mijailovich SM, Butler JP, Fredberg JJ, Stamenović D (2002) Cell prestress I: Stiffness and prestress are closely associated in adherent contractile cells. Am J Physiol Cell Physiol 282:C606–C616

    PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This study was supported by the Medical Scientific Research Foundation of China (Grant no. 10872140).

Conflict of interest

The authors have no financial or proprietary interest in any aspect of the study and have full control of all primary data, and we allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review our data if requested.

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Authors and Affiliations

  1. The Department of Ophthalmology, The Second Hospital of Hebei Medical University, Heping Road No.125, Shijiazhuang, 050000, Hebei Province, China

    Bo-Yu Chen & Jing-Xue Ma

  2. Department of Ophthalmology, Bethune International Peace Hospital of PLA, Shijiazhuang, 050082, China

    Bo-Yu Chen & Chao-Ying Wang

  3. Institute of Applied Mechanics & Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China

    Wei-Yi Chen

Authors
  1. Bo-Yu Chen
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  2. Jing-Xue Ma
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  3. Chao-Ying Wang
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  4. Wei-Yi Chen
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Correspondence to Jing-Xue Ma.

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Chen, BY., Ma, JX., Wang, CY. et al. Mechanical behavior of scleral fibroblasts in experimental myopia. Graefes Arch Clin Exp Ophthalmol 250, 341–348 (2012). https://doi.org/10.1007/s00417-011-1854-y

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  • DOI: https://doi.org/10.1007/s00417-011-1854-y

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