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IL-2 and IFN-gamma in the retina of diabetic rats

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Abstract

Background

The pathophysiology of the early events leading to diabetic retinopathy is not fully understood. It has been suggested that Inflammatory processes are involved in the development of the disease; however, the concentrations of tissue retinal inflammatory mediators and their possible alteration in diabetic retinopathy have not been described. The aim of this work was to study T-helper cell cytokine and chemokine profiles, and tyrosine nitration in retinal tissue of diabetic rats.

Methods

Cytokines (interleukin IL-1a, IL-1b, IL-2, IL-4, IL-6, IL-10, TNFa, GM-CSF, IFN-g), chemokines (MIP-1a, MIP-2, MIP-3a, MCP-1, GRO/KC, RANTES, Fractalkine), and tyrosine nitration were measured in retinal homogenate obtained from Long–Evans rats after 5 months of experimental diabetes.

Results

The T-helper type 1 cytokines IL-2 and INF-gamma, in addition to NO production (measured as nitrotyrosine), were found to be significantly elevated in diabetic rat retina homogenates. None of the other cytokines and chemokines studied were affected by the diabetic condition.

Conclusions

Immunoregulatory cytokines belonging to the Th-1 group (IL-2 and IFN-gamma) were increased in the retina of experimental diabetic rats. Moreover, the nitrotyrosine formation (as an expression of increased NO production) was significantly elevated in the diabetic retina, supporting the concept of an inflammatory element in the development of diabetic retinopathy.

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Authors and Affiliations

  1. Fundación Oftalmológica del Mediterráneo (FOM), Bifurcación Pio Baroja-General Avilés, s/n 46015, Valencia, Spain

    Siv Johnsen-Soriano, María Sancho-Tello, Emma Arnal, Amparo Navea, Francisco Bosch-Morell & Francisco Javier Romero

  2. Departamento de Fisiología, Farmacología y Toxicología, Universidad CEU-Cardenal Herrera, Avda Seminario s/n, Moncada, 46113, Valencia, Spain

    Siv Johnsen-Soriano, Francisco Bosch-Morell, Maria Miranda & Francisco Javier Romero

  3. Departamento de Patología, Facultad de Medicina y Odontología, Universidad de Valencia, Avda. Blasco Ibañez 15, 46010, Valencia, Spain

    María Sancho-Tello

  4. Servicio de Oftalmología, Hospital General Universitario, 46015, Valencia, Spain

    Enrique Cervera

Authors
  1. Siv Johnsen-Soriano
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  2. María Sancho-Tello
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  3. Emma Arnal
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  7. Maria Miranda
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Corresponding author

Correspondence to Francisco Javier Romero.

Additional information

A financial relationship with the Conselleria de Educación exists in form of a Grant. The authors have full control of all primary data, and we agree to allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review our data upon request.

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Johnsen-Soriano, S., Sancho-Tello, M., Arnal, E. et al. IL-2 and IFN-gamma in the retina of diabetic rats. Graefes Arch Clin Exp Ophthalmol 248, 985–990 (2010). https://doi.org/10.1007/s00417-009-1289-x

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  • DOI: https://doi.org/10.1007/s00417-009-1289-x

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