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Relevance of pupil size in the clinical determination of retinal straylight on young healthy human eyes

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To determine the impact of pupil size in the psychometric determination of intraocular straylight determined by the compensation comparison method in a population of young healthy adults.


Straylight was measured in 21 eyes from 11 young healthy subjects using the C-Quant (Oculus, Germany). Subjects were dilated with Phenylephrine 5%, and varying pupil size was simulated using three different diaphragms of 3, 5 and 6 mm in diameter. Three measurements were taken with each of the apertures in a randomized sequence. Mixed effects and repeated measures ANOVA was applied to test the significance of differences in straylight values for the fixed pupil diameters. Correlation analysis was applied to test relationships between the measurements for each fixed pupil diameter, and Bland–Altmann plots displayed the distribution of differences.


Significant differences between the straylight values obtained for the different fixed pupil diameters were not found. Correlation analysis did not show relationships between the values obtained for different diameters either (p > 0.05 in all cases). Bland–Altmann plots show that average differences are around zero regardless of the actual value measured, but there is also a substantial individual variation, with differences going as far up as 0.5 Log(s) units in some cases.


In healthy human eyes, straylight values measured with the Van den Berg straylightmeter are not affected significantly by the subject’s pupil size, although individual variations exist implying that no control for pupil size is required when estimating average amounts of retinal straylight from samples of eyes, but is desirable for the assessment of longitudinal variations of straylight in individual subjects, especially when assessing effects of surgical procedures and/or lens designs.

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  1. Wolf E, Gardiner JS (1965) Studies on the scatter of light in the dioptric media of the eye as a basis of visual glare. Arch Ophthalmol 74:338–345

    CAS  PubMed  Google Scholar 

  2. Vos JJ (2003) On the cause of disability glare and its dependence on glare angle, age and ocular pigmentation. Clin Exp Optom 86:363–370

    Article  PubMed  Google Scholar 

  3. van den Berg TJ (1991) On the relation between glare and straylight. Doc Ophthalmol 78:177–181

    Article  PubMed  Google Scholar 

  4. van den Berg TJ (1994) On the relation between intraocular straylight and visual function parameters. Invest Ophthalmol Vis Sci 35:2659–2661

    PubMed  Google Scholar 

  5. van den Berg TJ (1995) Analysis of intraocular straylight, especially in relation to age. Optom Vis Sci 72:52–59

    Article  PubMed  Google Scholar 

  6. van den Berg TJ, Tan KE (1994) Light transmittance of the human cornea from 320 to 700 nm for different ages. Vis Res 34:1453–1456

    Article  PubMed  Google Scholar 

  7. Veraart HG, van den Berg TJ, Hennekes R, Adank AM (1993) Stray light in photorefractive keratectomy for myopia. Bull Soc Belge Ophtalmol 249:57–62

    CAS  PubMed  Google Scholar 

  8. Harrison JM, Tennant TB, Gwin MC et al (1995) Forward light scatter at one month after photorefractive keratectomy. J Refract Surg 11:83–88

    CAS  PubMed  Google Scholar 

  9. Veraart HG, van den Berg TJ, Hennekes R, Adank AM (1995) Stray light in photorefractive keratectomy for myopia. Doc Ophthalmol 90:35–42

    Article  CAS  PubMed  Google Scholar 

  10. Elliott DB, Mitchell S, Whitaker D (1991) Factors affecting light scatter in contact lens wearers. Optom Vis Sci 68:629–633

    Article  CAS  PubMed  Google Scholar 

  11. Elliott DB, Fonn D, Flanagan J, Doughty M (1993) Relative sensitivity of clinical tests to hydrophilic lens-induced corneal thickness changes. Optom Vis Sci 70:1044–1048

    Article  CAS  PubMed  Google Scholar 

  12. van den Berg TJ, Coppens JE, van Best JA (2002) Derivation of lenticular transmittance from fluorophotometry. Invest Ophthalmol Vis Sci 43:3003–3007

    PubMed  Google Scholar 

  13. Coppens JE, Franssen L, van den Berg TJ (2006) Wavelength dependence of intraocular straylight. Exp Eye Res 82:688–692

    Article  CAS  PubMed  Google Scholar 

  14. Ijspeert JK, de Waard PW, van den Berg TJ, de Jong PT (1990) The intraocular straylight function in 129 healthy volunteers; dependence on angle, age and pigmentation. Vision Res 30:699–707

    Article  CAS  PubMed  Google Scholar 

  15. van den Berg TJ (1995) Analysis of intraocular straylight, especially in relation to age. Optom Vis Sci 72:52–59

    Article  PubMed  Google Scholar 

  16. Holladay JT, Van DH, Lang A et al (1990) Optical performance of multifocal intraocular lenses. J Cataract Refract Surg 16:413–422

    CAS  PubMed  Google Scholar 

  17. Cervino A, Hosking SL, Montes-Mico R, Alio JL (2008) Retinal straylight in patients implanted with monofocal and multifocal intraocular lenses. J Cataract Refract Surg 34:441–446

    Article  PubMed  Google Scholar 

  18. Hofmann T, Zuberbuhler B, Cervino A, Montés-Micó R, Haefliger E (2009) Retinal straylight and complaint scores 18 months after implantation of the AcrySof monofocal and ReSTOR diffractive intraocular lenses. J Refract Surg 25:485–492

    PubMed  Google Scholar 

  19. van den Berg TJ (1986) Importance of pathological intraocular light scatter for visual disability. Doc Ophthalmol 61:327–333

    Article  PubMed  Google Scholar 

  20. Franssen L, Coppens JE, van den Berg TJ (2006) Compensation comparison method for assessment of retinal straylight. Invest Ophthalmol Vis Sci 47:768–776

    Article  PubMed  Google Scholar 

  21. Coppens JE, Franssen L, van Rijn LJ, van den Berg TJ (2006) Reliability of the compensation comparison stray-light measurement method. J Biomed Opt 11:34027

    Article  PubMed  Google Scholar 

  22. van Rijn LJ, Nischler C, Gamer D et al (2005) Measurement of stray light and glare: comparison of Nyktotest, Mesotest, stray light meter, and computer implemented stray light meter. Br J Ophthalmol 89:345–351

    Article  PubMed  Google Scholar 

  23. Cerviño A, Montes-Mico R, Hosking SL (2008) Performance of the compensation comparison method for retinal straylight measurement: effect of patient's age on repeatability. Br J Ophthalmol 92(6):788–791

    Article  PubMed  Google Scholar 

  24. Cerviño A, Gonzalez-Meijome JM, Linhares JM, Hosking SL, Montés-Micó R (2008) Effect of sport-tinted contact lenses for contrast enhancement on retinal straylight measurements. Ophthalmic Physiol Opt 28(2):151–156

    Article  PubMed  Google Scholar 

  25. Ferrer-Blasco T, Montés-Micó R, Cerviño A, Alfonso JF (2009) Light scatter and disability glare after intraocular lens implantation. Arch Ophthalmol 127(4):576–577

    Article  PubMed  Google Scholar 

  26. Franssen L, Tabernero J, Coppens JE, van den Berg TJ (2007) Pupil size and retinal straylight in the normal eye. Invest Ophthalmol Vis Sci 48:2375–2382

    Article  PubMed  Google Scholar 

  27. Beerthuizen JJG, Franssen L, Landesz M, van den Berg TJTP (2007) Straylight values 1 month after laser in situ keratomileusis and photorefractive keratectomy. J Cataract Refract Surg 33:779–783

    Article  PubMed  Google Scholar 

  28. Veraart HG, van den Berg TJ, IJspeert JK, Cardozo OL (1992) Stray light in radial keratotomy and the influence of pupil size and straylight angle. Am J Ophthalmol 114:424–428

    CAS  PubMed  Google Scholar 

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Correspondence to Alejandro Cerviño.

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The authors have no proprietary or financial interest in the instrument used in this study.

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García-Lázaro, S., Ferrer-Blasco, T., Ortí-Navarro, S. et al. Relevance of pupil size in the clinical determination of retinal straylight on young healthy human eyes. Graefes Arch Clin Exp Ophthalmol 248, 395–399 (2010).

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