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Splenic CD8+ T cells secrete TGF-β1 to exert suppression in mice with anterior chamber-associated immune deviation

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Abstract

Background

CD8+ regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8+ T cells in ACAID remain only poorly understood. TGF-β1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-β1 by CD8+ T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-β1, is unknown.

Methods

The suppressive effect of CD8+ T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-β1 by CD8+ T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-β1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8+ T cells.

Results

CD8+ T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-β1, and their suppression could partially be blocked by anti-TGF-β1 antibodies.

Conclusions

Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-β1.

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Acknowledgement

This study is supported by Project of International Cooperation in Science and Technology, Guangdong Province (2004B50301002, 2006A50107001); Key Project of National Natural Science Foundation (30630064); National Supporting Project of P.R. China (2007BAI18B10).

Declaration

All the listed authors have participated actively in the study, and have read and approved the submitted manuscript. None of the authors has any potential financial conflict of interest related to this manuscript.

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Authors and Affiliations

  1. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Guangzhou, People’s Republic of China

    Liqiong Jiang, Hao He, Xiaomin Lin, Hongyan Zhou & Xiangkun Huang

  2. The First Affiliated Hospital, Chongqing Medical University, Youyi Road 1, Chongqing, 400016, People’s Republic of China

    Peizeng Yang

  3. Department of Ophthalmology, Eye Research Institute Maastricht, University Hospital Maastricht, Maastricht, The Netherlands

    Aize Kijlstra

  4. Animal Sciences Group, Wageningen University, Lelystad, The Netherlands

    Aize Kijlstra

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  1. Liqiong Jiang
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  2. Hao He
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  3. Peizeng Yang
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  4. Xiaomin Lin
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  5. Hongyan Zhou
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Correspondence to Peizeng Yang.

Additional information

Liqiong Jiang and Hao He contributed equally to this work.

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Jiang, L., He, H., Yang, P. et al. Splenic CD8+ T cells secrete TGF-β1 to exert suppression in mice with anterior chamber-associated immune deviation. Graefes Arch Clin Exp Ophthalmol 247, 87–92 (2009). https://doi.org/10.1007/s00417-008-0947-8

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  • DOI: https://doi.org/10.1007/s00417-008-0947-8

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