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Immune privilege of allogeneic neuroretinal transplants in the subconjunctival space

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Abstract

Background

The extent of site and tissue-associated immune privilege is of great interest in transplantation experiments involving the CNS. In the present paper we have explored neuroretinal immune privilege by transplantation to a non-immune privileged site.

Methods

Fetal and adult full-thickness rabbit neuroretinal grafts were placed in the subconjunctival space of immunocompetent rabbit hosts. Morphological examination was performed after 2–31 days (fetal grafts, n = 46), and after 8 days (adult grafts, n = 4).

Results

Hematoxylin and eosin-stained sections and immunohistochemistry directed against microtubule-associated protein 2 (MAP2) revealed surviving grafts containing retinal neurons in the majority of eyes with fetal grafts. In all specimens, a mild inflammatory reaction was evident as seen with major histocompatibility complex class II (MHC-II) labeling. Short-term grafts survived well and displayed lamination and rosette formation whereas older grafts appeared more disorganized and were more often rejected. Müller cell fibers labeled with glial fibrillary acidic protein (GFAP) were present in grafts from 15 days and onwards. Adult grafts were destroyed after 8 days.

Conclusions

Allogeneic fetal full-thickness neuroretinal transplants can survive for several weeks in a non-immune privileged environment in which adult grafts are rapidly rejected. Fetal grafts gradually shrink, lose their architecture and go through a glial transformation accompanied by low-grade inflammation. The rabbit neuroretina thus appears to enjoy partial immune privilege, the extent of which depends on the development state of the tissue. The characterization of neuroretinal immune privilege will hopefully influence future clinical trials of retinal transplantation.

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Authors and Affiliations

  1. Department of Ophthalmology, University Hospital, 22185, Lund, Sweden

    Fredrik Ghosh, Ola Rauer & Karin Arnér

Authors
  1. Fredrik Ghosh
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  2. Ola Rauer
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  3. Karin Arnér
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Corresponding author

Correspondence to Fredrik Ghosh.

Additional information

Supported by the Faculty of Medicine, Lund University, the Crown Princess Margaret Committee for the Blind, the Swedish Research Council. The authors have no financial relationship with the sponsoring organizations.

The authors have full control of all primary data and agree to allow Graefe’s Archive for Clinical and Experimental Ophthalmology to review the data if requested.

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Ghosh, F., Rauer, O. & Arnér, K. Immune privilege of allogeneic neuroretinal transplants in the subconjunctival space. Graefes Arch Clin Exp Ophthalmol 246, 1715–1722 (2008). https://doi.org/10.1007/s00417-008-0933-1

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  • DOI: https://doi.org/10.1007/s00417-008-0933-1

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