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Microphthalmia and lack of vitreous body in transgenic mice expressing the first immunoglobulin-like domain of nectin-1

  • Basic Science
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Abstract

Background

Nectins are Ca2+-independent immunoglobulin (Ig)-like cell-cell-adhesion molecules. We have generated transgenic mice expressing a series of soluble forms of nectin-1, and investigated special effects of each soluble form of nectin-1 in vivo. In the course of generating transgenic mice expressing a soluble form of nectin-1 consisting of the first Ig-like domain of nectin-1 and the Fc portion of human IgG1 (PHveC-VhIg), we found that all of the transgenic founder mice showed a microphthalmia. The purpose of this study is to examine functions of the extracellular domains of nectin-1 in eye development using transgenic technology.

Methods

Eyes of four different transgenic mouse lines expressing each soluble form of nectin-1 were analyzed histologically. Tissue sections were processed with hematoxylin-eosin staining and indirect immunoperoxidase technique.

Results

All of five transgenic mouse founders expressing PHveC-VhIg, and of three lines expressing PHveC-VpIg made of the first Ig-like domain fused to porcine Fc portions at 5 weeks showed a microphthalmia, but not all of the transgenic mouse lines expressing PHveCIg or PHveCpIg made of the entire ectodomain fused to human or porcine Fc portions. In the abnormal eyes, the vitreous body was almost absent. In PHveC-VhIg-expressing mice at postnatal day 6, each vitreous space was very small. In the neonatal transgenic mice, the vitreous body was almost the same as that of control mice, and PHveC-VhIg was expressed in the optic nerve, conjunctival epithelium, ciliary body, corneal and lens epithelium. At this stage, nectin-1, -3 and -4 were stained in the optic nerve of control mice as well as in that of the transgenic mice. Nectin-1 is faintly stained in the epithelium of the cornea and lens epithelium, but not in the ciliary body.

Conclusion

Soluble forms of the first Ig-like domain of nectin-1 (PHveC-VhIg and PHveC-VpIg), but not those of the entire ectodomain (PHveCIg and PHveCpIg), lead to microphthalmia and lack of vitreous body in the transgenic mice.

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Acknowledgements

This study was supported by Grants-in-Aid for Scientific Research (C)(2) from The Ministry of Education, Culture, Sports, Science and Technology, Japan. We thank Dr. J. Miyazaki for pCXN2.

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Authors and Affiliations

  1. Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, North 15, West 7, Kita-ku, Sapporo, 060-8638, Japan

    Kazuhiko Yoshida, Satoru Kase & Shigeaki Ohno

  2. Laboratory of Animal Experiments for Disease Model, Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-0815, Japan

    Yukiko Tomioka, Masami Morimatsu & Etsuro Ono

  3. The Avian Zoonoses Research Centre, Faculty of Agriculture, Tottori University, Tottori, 680-8553, Japan

    Kyoko Shinya & Etsuro Ono

  4. Laboratory of Biomedicine, Center of Biomedical Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan

    Etsuro Ono

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  1. Kazuhiko Yoshida
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  2. Yukiko Tomioka
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  3. Satoru Kase
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  4. Masami Morimatsu
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  5. Kyoko Shinya
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  6. Shigeaki Ohno
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  7. Etsuro Ono
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Transgenic mice generating group

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Correspondence to Etsuro Ono.

Additional information

Transgenic mice generating group: Keiko Amagai, Minako Kuramochi, Yuki Watanabe and Shigeto Kouda (Sankyo Labo Service Corporation, Tokyo 132-0023, Japan).

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Yoshida, K., Tomioka, Y., Kase, S. et al. Microphthalmia and lack of vitreous body in transgenic mice expressing the first immunoglobulin-like domain of nectin-1. Graefes Arch Clin Exp Ophthalmol 246, 543–549 (2008). https://doi.org/10.1007/s00417-007-0750-y

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  • DOI: https://doi.org/10.1007/s00417-007-0750-y

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