Abstract
Background
Behçet disease (BD) is manifested by recurrent acute iridocyclitis with hypopyon in the active phase, which regresses spontaneously. Hypopyon consists of inflammatory cells infiltrating the eye, with polymorphonuclear cells (PMNs) as the main component. The present study was conducted to investigate the apoptosis property of PMNs in BD patients with uveitis.
Methods
PMNs were purified from peripheral blood cells of BD patients with uveitis in the active or remission phase and were cultured for 12 hours. In some cultures, lipopolysaccharide (LPS), antagonistic anti-TNFα antibody, agonistic anti-Fas antibody, or Fas:Fc fusion protein was added. At the end of cultures, apoptotic cells were evaluated by Annexin V expression using flow cytometry.
Results
Spontaneous apoptosis of PMNs showed lower levels in the remission phase of BD-related uveitis compared with the active phase or healthy controls. The lower level of PMN apoptosis in the remission phase of uveitis in BD remained even by stimulation with LPS, anti-TNFα antibody, or Fas:Fc fusion protein, which was abolished in the presence of agonistic anti-Fas antibody.
Conclusions
In BD patients, the apoptosis of PMNs was reduced in the remission phase of uveitis and restored in the active phase, which arose from the apoptotic cell death in part via Fas-Fas ligand interaction.
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Acknowledgements
This work was supported by Grant-in-Aid 17791258 for Scientific Research from the Japan Society for the Promotion of Science. We thank Kazumi Nagasawa for technical assistance in analyzing the apoptosis assay.
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Keita Fujimori and Keiko Oh-i contributed equally to this study.
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Fujimori, K., Oh-i, K., Takeuchi, M. et al. Circulating neutrophils in Behçet disease is resistant for apoptotic cell death in the remission phase of uveitis. Graefes Arch Clin Exp Ophthalmol 246, 285–290 (2008). https://doi.org/10.1007/s00417-007-0659-5
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DOI: https://doi.org/10.1007/s00417-007-0659-5