Skip to main content

Reelin expression is upregulated following ocular tissue injury

Graefe's Archive for Clinical and Experimental Ophthalmology volume 245, pages 889–893 (2007)Cite this article

Abstract

Purpose

Reelin is important in the guidance of neuronal stem cells in the central nervous system during normal development. We wished to determine whether reelin is expressed in the retina and cornea after injury.

Methods

Mice underwent laceration of their retina as well as corneal epithelial debridement. The mice were sacrificed at 3 days, and eyes were fixed and stained for reelin expression and reelin messenger ribonucleic acid (mRNA).

Results

In normal eyes, reelin was expressed only at very low levels in the ganglion cell layer of the retina and the endothelial cell layer of the cornea. In injured eyes, there was marked expression in reelin immunoreactivity in the retina and cornea. Reelin gene expression was seen in the retina and cornea.

Conclusions

Reelin is expressed during normal retinogenesis. This study shows that reelin is also upregulated following injury to the retina and cornea. The expression of reelin following injury suggests that reelin may play an important role in regulating stem cell trafficking in neuronal and nonneuronal tissues following injury similar to its role in normal organogenesis.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2
Fig. 3

References

  1. Abraham H, Meyer G (2003) Reelin-expressing neurons in the postnatal and adult human hippocampal formation. Hippocampus 13:715–727

    PubMed  Article  CAS  Google Scholar 

  2. Alcantara S, Ruiz M, D’Arcangelo G, Ezan F, de Lecea L, Curran T, Sotelo C, Soriano E (1998) Regional and cellular patterns of reelin mRNA expression in the forebrain of the developing and adult mouse. J Neurosci 18:7779–7799

    PubMed  CAS  Google Scholar 

  3. D’Arcangelo G, Miao GG, Chen SC, Soares HD, Morgan JI, Curran T (1995) A protein related to extracellular matrix proteins deleted in the mouse mutant reeler. Nature 374:719–723

    PubMed  Article  CAS  Google Scholar 

  4. de Bergeyck V, Naerhuyzen B, Goffinet AM, Lambert de Rouvroit C (1998) A panel of monoclonal antibodies against reelin, the extracellular matrix protein defective in reeler mutant mice. J Neurosci Methods 82:17–24

    PubMed  Article  Google Scholar 

  5. Dong X, Pulido JS, Qu T, Sugaya K (2003) Differentiation of human neural stem cells into retinal cells. Neuroreport 14:143–146

    PubMed  Article  CAS  Google Scholar 

  6. Kim HM, Qu T, Kriho V, Lacor P, Smalheiser N, Pappas GD, Guidotti A, Costa E, Sugaya K (2002) Reelin function in neural stem cell biology. Proc Natl Acad Sci USA 99:4020–4025

    PubMed  Article  CAS  Google Scholar 

  7. Leavitt BR, Hernit-Grant CS, Macklis JD (1999) Mature astrocytes transform into transitional radial glia within adult mouse neocortex that supports directed migration of transplanted immature neurons. Exp Neurol 157:43–57

    PubMed  Article  CAS  Google Scholar 

  8. Qu T, Brannen CL, Kim HM, Sugaya K (2001) Human neural stem cells improve cognitive function of aged brain. Neuroreport 12:1127–1132

    PubMed  Article  CAS  Google Scholar 

  9. Smalheiser NR, Costa E, Guidotti A, Impagnatiello F, Auta J, Lacor P, Kriho V, Pappas GD (2000) Expression of reelin in adult mammalian blood, liver, pituitary pars intermedia, and adrenal chromaffin cells. Proc Natl Acad Sci USA 97:1281–1286

    PubMed  Article  CAS  Google Scholar 

  10. Wolosin JM, Xiong X, Schutte M, Stegman Z, Tieng A (2000) Stem cells and differentiation stages in the limbo-corneal epithelium. Prog Retin Eye Res 19:223–255

    PubMed  Article  CAS  Google Scholar 

Download references

Acknowledgements

This study was partially supported by an unrestricted grant from Research to Prevent Blindness (University of Illinois, Chicago and Mayo Clinic, Rochester), New York, a Core Grant from the National Eye Institute (EY01792), a grant from the McGraw Foundation, National Institute on Aging (AG 23472), and a grant from Mrs. Charlotte Simmons. We also are grateful to Ms. Christine Pulido and Mr. Andrew Sugaya for their assistance in the experimental aspects of the study.

Author information

Affiliations

  1. Department of Ophthalmology and Visual Sciences, University of Illinois at Chicago, Chicago, IL, 60612, USA

    Jose S. Pulido & Kiminobu Sugaya

  2. Department of Psychiatry, University of Illinois at Chicago, Chicago, IL, 60612, USA

    Jordan Comstock & Kiminobu Sugaya

  3. Biomolecular Science Center, University of Central Florida, 4000 Central Florida Blvd., BMS building, Room 223, Orlando, FL, 32816-2364, USA

    Ikuko Sugaya & Kiminobu Sugaya

  4. Department of Ophthalmology, Mayo Clinic, Rochester, MN, 55905, USA

    Jose S. Pulido

Authors
  1. Jose S. Pulido
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ikuko Sugaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jordan Comstock
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kiminobu Sugaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kiminobu Sugaya.

Additional information

For consideration of publication in Graefe’s Archive for Clinical and Experimental Ophthalmology.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Pulido, J.S., Sugaya, I., Comstock, J. et al. Reelin expression is upregulated following ocular tissue injury. Graefe's Arch Clin Exp Ophthalmol 245, 889–893 (2007). https://doi.org/10.1007/s00417-006-0458-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00417-006-0458-4

Keywords

  • Cornea
  • Migration
  • Regeneration
  • Retina
  • Stem cell
  • Reelin