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Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits

  • Laboratory Investigation
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Abstract

Background

Dopamine (DA) storage and release are reduced in form deprivation myopia (FDM) in a wide range of species, from chicks to primates. FDM can be prevented by treatment with DA agonists such as apomorphine, and paradoxically by the dopamine neurotoxin 6-hydroxydopamine. In this study, we increased the DA levels by direct intravitreal DA injections to learn if FDM can be suppressed in a rabbit model.

Methods

Seven-day-old rabbits were deprived of pattern vision by the suturing the right eyelids after natural eye opening. In the first group (FD, n=20), the right eye received form deprivation (FD) alone. In the second group (DA-FD, n=16), the deprived eye of 7-day-old rabbits received four intravitreal injections of 20 μg dopamine every 5 days. In the third group (saline-FD, n=16), the deprived eye received saline injections with the same schedule. The contralateral eye remained untreated as a control. At the end of the 8-week deprivation period, the effects of DA on refractive error, corneal curvature and ocular dimensions were assessed by streak retinoscopy, keratometry and A-scan ultrasonography, respectively.

Results

Eight weeks of FDM induced a myopic shift of −2.70±0.87 D (n=20) in treated eyes compared with contralateral eyes. The major structural correlate of the myopia appeared to be elongation of the vitreous chamber (0.7±0.3 mm, n=20) and axial elongation (0.9±0.3 mm, n=20), respectively. Repeated intravitreal injections of DA fully prevented the myopic shift (−0.06±0.37 D), elongation of the vitreous chamber (0.1±0.3 mm, n=16) and axial elongation (0.3±0.2 mm, n=16) due to lid suture, whereas saline injections had slight effect.

Conclusions

FD by suturing eyelids is an effective technique to induce a significant myopic shift, vitreous chamber and axial elongation in rabbits as a model of myopia development. These changes associated with FD were retarded by intravitreal injections of DA.

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Acknowledgements

We would like to thank Dr. Jojo W. Du at SUNY State College of Optometry, for her through reading and constructive revision of the manuscript. Her invaluable comments and suggestions on this paper are gratefully acknowledged. This study was supported by the National Nature Science Foundation of China (30200306), the Science and Technology Foundation of Guang Dong Province of China (2003C32715) and the Science and Technology Foundation of Guang Zhou City of China (2002J1-C0381).

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Authors and Affiliations

  1. Key Laboratory of Ophthalmology, Ministry of Education, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, 510060, People’s Republic of China

    Qianying Gao, Quan Liu, Ping Ma, XingWu Zhong, Junshu Wu & Jian Ge

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  1. Qianying Gao
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Correspondence to Jian Ge.

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Gao, Q., Liu, Q., Ma, P. et al. Effects of direct intravitreal dopamine injections on the development of lid-suture induced myopia in rabbits. Graefe's Arch Clin Exp Ophthalmo 244, 1329–1335 (2006). https://doi.org/10.1007/s00417-006-0254-1

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