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Vitreous collagen metabolism before and after vitrectomy

  • Clinical Investigation
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Abstract

Purpose

To assess vitreous metabolism by measuring C-propeptide levels of type II procollagen (pCOL-II-C) and hyaluronan levels in the vitreous and in the vitreous fluid after vitrectomy for macular hole.

Methods

We obtained 1-ml vitreous samples during vitrectomy from 34 patients (34 eyes) with a macular hole (age range 50–77 years, mean 64 years). After vitrectomy, we performed fluid–air exchange in six eyes because of unresolved macular holes and collected 4-ml fluid samples. Gel-filtration high-performance liquid chromatography (HPLC) was used to determine the molecular weight of pCOL-II-C in the samples. The pCOL-II-C level was measured by sandwich enzyme immunoassay and hyaluronan by sandwich binding protein assay.

Results

HPLC showed that pCOL-II-C in the vitreous samples corresponded to purified pCOL-II-C from cartilage. The vitreous pCOL-II-C level (4.7±0.3 ng/ml) was similar to reported synovial fluid levels. In six eyes that underwent fluid–air exchange, pCOL-II-C in the fluid samples remained at a level similar to that in the vitreous samples, while hyaluronan levels in the fluid samples were significantly lower than in the vitreous samples.

Conclusions

The molecular weight and concentrations of pCOL-II-C in the vitreous are similar to those in joint fluid. In patients with a macular hole, type II procollagen may be secreted persistently into the vitreous cavity before and after vitrectomy.

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Acknowledgement

Supported in part by a grant-in-aid for scientific research from the Ministry of Education, Science, Sport and Culture of Japan (SK).

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Authors and Affiliations

  1. Department of Ophthalmology, Gunma University School of Medicine, 3-39-15 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Hirotaka Itakura & Shoji Kishi

  2. Department of Laboratory Medicine, Gunma University School of Medicine, Gunma, Japan

    Nobuo Kotajima & Masami Murakami

Authors
  1. Hirotaka Itakura
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  2. Shoji Kishi
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  3. Nobuo Kotajima
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  4. Masami Murakami
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Correspondence to Shoji Kishi.

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Itakura, H., Kishi, S., Kotajima, N. et al. Vitreous collagen metabolism before and after vitrectomy. Graefe's Arch Clin Exp Ophthalmo 243, 994–998 (2005). https://doi.org/10.1007/s00417-005-1150-9

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  • DOI: https://doi.org/10.1007/s00417-005-1150-9

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