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Expression of estrogen receptor α and 17β-hydroxysteroid dehydrogenase 4 in the ciliary body

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Abstract

Purpose

To investigate the expression and distribution of estrogen receptor α (ERα) and 17β-hydroxysteroid dehydrogenase 4 (HSD4) in bovine and monkey ciliary body.

Methods

Immunohistochemical study was employed to investigate the expression of ERα and 17β-HSD4. To detect ERα and 17β-HSD4 transcripts, reverse transcription–polymerase chain reaction (RT-PCR) was used.

Results

In bovine and monkey ciliary body, the staining of the anti-ERα antibody was distributed in nonpigmented epithelium, and was not detectable in pigmented epithelium. Vessels in the stroma were also stained. The expression of 17β-HSD4 was observed in nonpigmented and pigmented epithelium. RT-PCR revealed that the transcripts of ERα and 17β-HSD4 were expressed in monkey ciliary body.

Conclusions

This study provides evidence for the presence of ERα and 17β- HSD4 in the ciliary body. The presence of ERα and 17β-HSD4 suggests that estrogen may have important functions in the ciliary body.

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Fig. 1 A–D
Fig. 2 A–C
Fig. 3 A,B

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Authors and Affiliations

  1. Department of Ophthalmology, Saga Medical School, 5-1-1 Nabeshima, 849-8501, Saga , Japan

    Kaori Kobayashi, Ryo Iwakiri, Hiroshi Kobayashi & Satoshi Okinami

Authors
  1. Kaori Kobayashi
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  2. Ryo Iwakiri
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  3. Hiroshi Kobayashi
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  4. Satoshi Okinami
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Correspondence to Kaori Kobayashi.

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Kobayashi, K., Iwakiri, R., Kobayashi, H. et al. Expression of estrogen receptor α and 17β-hydroxysteroid dehydrogenase 4 in the ciliary body. Graefe's Arch Clin Exp Ophthalmol 242, 172–176 (2004). https://doi.org/10.1007/s00417-003-0799-1

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  • DOI: https://doi.org/10.1007/s00417-003-0799-1

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