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Expression of serotonin receptor mRNAs in human ciliary body: a polymerase chain reaction study

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Abstract

Background

Previous research has indicated a role for serotonin (5-HT) in the anterior uvea of the eye. The purpose of this study was to examine whether mRNAs encoding particular 5-HT receptors are expressed in the ciliary body and iris of a number of human subjects.

Methods

The presence of mRNA encoding 5-HT receptors in four human ciliary body samples was determined by reverse transcription–polymerase chain reaction experiments using a standard methodology.

Results

Positive signals for 5-HT1A, 5-HT2A, 5-HT2B, 5-HT2C and 5-HT7 receptor mRNAs were detected in the samples prepared from various human ciliary body samples.

Conclusion

The detection of certain 5-HT receptor mRNAs in the human ciliary body supports the hypothesis that serotonin is involved in the control of aqueous dynamics and indicates that ligands acting on these 5-HT receptors may have potential use as intraocular pressure-lowering agents.

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Acknowledgements

The authors acknowledge the assistance of Prof. B.E. Damato and the financial support of the European Union (PRO-AGE-RET).

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Authors and Affiliations

  1. Nuffield Laboratory of Ophthalmology, University of Oxford, Walton Street, Oxford, UK

    Glyn Chidlow & Neville N. Osborne

  2. Unit of Ophthalmology, Department of Medicine, University of Liverpool, Liverpool, UK

    Paul S. Hiscott

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  1. Glyn Chidlow
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  2. Paul S. Hiscott
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  3. Neville N. Osborne
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Correspondence to Glyn Chidlow.

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Chidlow, G., Hiscott, P.S. & Osborne, N.N. Expression of serotonin receptor mRNAs in human ciliary body: a polymerase chain reaction study. Graefe's Arch Clin Exp Ophthalmol 242, 259–264 (2004). https://doi.org/10.1007/s00417-003-0785-7

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  • DOI: https://doi.org/10.1007/s00417-003-0785-7

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