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Calcium antagonists in N-methyl d-aspartate-induced retinal injury

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Abstract

Purpose

To detect the neuroprotective role of nimodipine and dantrolene in N-methyl-d-aspartate (NMDA)-induced retinal injury.

Methods

In this study we used two calcium antagonists which have two different modes of action, nimodipine and dantrolene, to prevent NMDA-induced retinal ischemia in guinea pigs. In 40 animals we injected nimodipine (n=10), dantrolene (n=10), a combination of both (n=10) or sterile 0.9% NaCl solution as a placebo (n=10) before intravitreal injection of NMDA. We enucleated one eye of each animal after 48 hours and performed histopathologic examination. We also measured malondialdehyde (MDA) levels in retinal homogenates as a marker of ischemic injury.

Results

Our results indicate that ganglion cells of the retina were preserved mostly by nimodipine, followed by combined nimodipine–dantrolene, and dantrolene respectively. The ganglion cell count was statistically significantly higher in cases where we used calcium antagonists than in the control group (p<0.05). We also found that MDA was significantly reduced by calcium antagonists compared to the control group (p<0.05).

Conclusion

Our data show that nimodipine and dantrolene both have potential neuroprotective effects; nimodipine preserved retinal ganglion cells to a greater extent than dantrolene from NMDA-induced retinal injury.

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Authors and Affiliations

  1. Department of Ophthalmology, Abant Izzet Baysal University Düzce Medical School, Konuralp-Düzce, Turkey

    Murat Kaya & Murat Tunç

  2. Department of Ophthalmology, Atatürk University Medical School, Erzurum, Turkey

    Talha Özdemir

  3. Department of Biochemistry, Suleyman Demirel University Medical School, Isparta, Turkey

    İrfan Altuntaş

Authors
  1. Murat Kaya
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  2. Murat Tunç
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  3. Talha Özdemir
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  4. İrfan Altuntaş
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Correspondence to Murat Kaya.

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Kaya, M., Tunç, M., Özdemir, T. et al. Calcium antagonists in N-methyl d-aspartate-induced retinal injury. Graefe's Arch Clin Exp Ophthalmol 241, 418–422 (2003). https://doi.org/10.1007/s00417-002-0610-8

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  • DOI: https://doi.org/10.1007/s00417-002-0610-8

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