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Antioxidant capacity and protein oxidation in cerebrospinal fluid of amyotrophic lateral sclerosis

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Abstract

Background

The causes of Amyotrophic Lateral Sclerosis (ALS) are unknown. A bulk of evidence supports the hypothesis that oxidative stress and mitochondrial dysfunction can be implicated in ALS pathogenesis.

Methods

We assessed, in cerebrospinal fluid (CSF) and in plasma of 49 ALS patients and 8 controls, the amount of oxidized proteins (AOPP, advanced oxidation protein products), the total antioxidant capacity (FRA, the ferric reducing ability), and, in CSF, two oxidation products, the 4-hydroxynonenal and the sum of nitrites plus nitrates.

Results

The FRA was decreased (p = 0.003) in CSF, and AOPP were increased in both CSF (p = 0.0039) and plasma (p = 0.001) of ALS patients. The content of AOPP was differently represented in CSF of ALS clinical subsets, resulting in increase in the common and pseudopolyneuropathic forms (p < 0.001) and nearly undetectable in the bulbar form, as in controls. The sum of nitrites plus nitrates and 4-hydroxynonenal were unchanged in ALS patients compared with controls.

Conclusion

Our results, while confirming the occurrence of oxidative stress in ALS, indicate how its effects can be stratified and therefore implicated differently in the pathogenesis of different clinical forms of ALS.

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Authors and Affiliations

  1. Dept. of Neuroscience Neurological clinic, University of Pisa, Via Roma 67, 56126, Pisa, Italy

    G. Siciliano MD, PhD, S. Piazza MD, C. Carlesi MD, A. Del Corona MD, M. Mancuso MD, PhD & L. Murri MD

  2. Dept. of Experimental Pathology, University of Pisa, Pisa, Italy

    M. Franzini PhD, A. Pompella MD, G. Malvaldi MD & A. Paolicchi MD

Authors
  1. G. Siciliano MD, PhD
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  2. S. Piazza MD
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  3. C. Carlesi MD
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  4. A. Del Corona MD
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  5. M. Franzini PhD
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  6. A. Pompella MD
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  7. G. Malvaldi MD
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  8. M. Mancuso MD, PhD
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  9. A. Paolicchi MD
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  10. L. Murri MD
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Correspondence to G. Siciliano MD, PhD.

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Siciliano, G., Piazza, S., Carlesi, C. et al. Antioxidant capacity and protein oxidation in cerebrospinal fluid of amyotrophic lateral sclerosis. J Neurol 254, 575–580 (2007). https://doi.org/10.1007/s00415-006-0301-1

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  • DOI: https://doi.org/10.1007/s00415-006-0301-1

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