International Journal of Legal Medicine

, Volume 115, Issue 2, pp 64–69 | Cite as

Detecting errors in mtDNA data by phylogenetic analysis

  • H.-J. Bandelt
  • P. Lahermo
  • M. Richards
  • V. Macaulay
Original article

Abstract

Sequencing and documenting a sample of homologous DNA stretches is prone to copying errors in a way rather analogous to the biological replication process. Previous attempts at obtaining representative mtDNA sequences, typically of the control region, for evolutionary studies or forensic purposes have yielded rather unsatisfactory results in many cases. The key ingredient in pinpointing problems with given data is the phylogenetic analysis of closely related mtDNAs within the framework of an established worldwide phylogeny that is supported by coding region information. We develop some general rules by which likely errors in data tables can readily be detected without rereading whole sequences repeatedly. Following these guidelines, one can expect to lower the error rate by at least an order of magnitude, although it will still be hard to beat the mitochondrial gamma polymerase in precision.

Keywords Mitochondrial DNA Error detection Sequence data Phylogenetic analysis Types of error 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • H.-J. Bandelt
    • 1
  • P. Lahermo
    • 2
  • M. Richards
    • 3
  • V. Macaulay
    • 4
  1. 1.Fachbereich Mathematik, Universität Hamburg, 20146 Hamburg, GermanyDE
  2. 2.Finnish Genome Center, University of Helsinki, 00014 Helsinki, FinlandFI
  3. 3.Department of Chemical and Biological Sciences, University of Huddersfield, Huddersfield, UKGB
  4. 4.Department of Statistics, University of Oxford, 1 South Parks Road, Oxford, OX1 3TG, UK e-mail: macaulay@stats.ox.ac.uk, Fax: +44-1865-272595GB

Personalised recommendations