Table 5 Robustness of MiRNA isolated using the Bioneer AccuPrep® genomic DNA extraction kit, Qiagen QIAamp DNA mini kit, and Qiagen MiRNeasy Tissue/Cells advanced micro kit from human saliva samples of 400 µL, 200 µL, 100 µL, 50 µL, and 25 µL
Robustness of miRNA Yield | ||||
|---|---|---|---|---|
Extraction Method | Sample Volume (µL) | CV% | Acceptance | P-value |
Genomic DNA Kit | 400 | 4.91% | CV < 5% | < 0.0001 |
200 | 1.71% | CV < 5% | ||
100 | 14.78% | CV < 20% | ||
50 | 7.53% | CV < 10% | ||
25 | 23.45% | CV < 30% | ||
QIAamp DNA Kit | 400 | 5.53% | CV < 10% | 0.21 |
200 | 11.68% | CV < 20% | ||
100 | 2.99% | CV < 5% | ||
50 | 54.84% | CV > 30% | ||
25 | 11.26% | CV < 20% | ||
miRNeasy Tissue Kit | 400 | 8.82% | CV < 10% | < 0.001 |
200 | 22.22% | CV < 30% | ||
100 | 9.85% | CV < 10% | ||
50 | 2.84% | CV < 5% | ||
25 | 19.73% | CV < 20% | ||