Table 4 Robustness and purity of RNA isolated using the Bioneer AccuPrep® genomic DNA extraction kit, Qiagen QIAamp DNA mini kit, and Qiagen MiRNeasy Tissue/Cells advanced micro kit from human saliva samples of 400 µL, 200 µL, 100 µL, 50 µL, and 25 µL
Robustness of RNA Yield | RNA Purity (A260nm/A280nm ratio) | RNA Purity(A260nm/A230nm ratio) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Extraction Method | Sample Volume (µL) | CV% | Acceptance | P-value | Mean | SD | P-value | Mean | SD | P-value |
Genomic DNA Kit | 400 | 8.56% | CV < 10% | < 0.0001 | 1.86 | 0.01 | 0.12 | 2.05 | 0.03 | 0.03 |
200 | 3.84% | CV < 5% | 1.90 | 0.03 | 1.86 | 0.24 | ||||
100 | 0.62% | CV < 5% | 1.70 | 0.39 | 1.09 | 0.16 | ||||
50 | 1.49% | CV < 5% | 2.55 | 0.22 | 1.10 | 0.25 | ||||
25 | 16.54% | CV < 20% | 1.72 | 0.83 | 0.68 | 0.12 | ||||
QIAamp DNA Kit | 400 | 4.69% | CV < 5% | <0.001 | 2.27 | 0.15 | 0.72 | 1.66 | 0.45 | 0.72 |
200 | 9.61% | CV < 10% | 1.79 | 0.31 | 1.78 | 0.59 | ||||
100 | 4.59% | CV < 5% | 2.44 | 0.30 | 1.09 | 0.68 | ||||
50 | 9.75% | CV < 10% | 2.24 | 0.41 | 1.99 | 0.68 | ||||
25 | 20.86% | CV < 30% | 1.90 | 0.37 | 1.02 | 0.56 | ||||
miRNeasy Tissue Kit | 400 | 1.74% | CV < 5% | < 0.0001 | 2.05 | 0.15 | 0.68 | 0.14 | 0.04 | 0.05 |
200 | 15.96% | CV < 20% | 2.00 | 0.10 | 0.05 | 0.02 | ||||
100 | 9.44% | CV < 10% | 2.18 | 0.29 | 0.04 | 0.02 | ||||
50 | 6.06% | CV < 10% | 2.41 | 0.07 | 0.03 | 0.01 | ||||
25 | 12.37% | CV < 20% | 2.09 | 0.37 | 0.10 | 0.02 | ||||