Table 3 Robustness and purity of DNA isolated using Bioneer AccuPrep® Genomic DNA Extraction Kit, Qiagen QIAamp DNA Mini Kit, and Qiagen miRNeasy Tissue/Cells Advanced Micro Kit from human saliva samples of 400 μL, 200 μL, 100 μL, 50 μL, and 25 μL
Robustness of DNA Yield | DNA Purity (A260nm/A280nm ratio) | DNA Purity (A260nm/A230nm ratio) | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Extraction Method | Sample Volume (µL) | CV% | Acceptance | P-value | Mean | SD | P-value | Mean | SD | P-value | ||
Genomic DNA Kit | 400 | 6.79% | CV < 10% | < 0.0001 | 1.86 | 0.03 | 0.29 | 1.80 | 0.15 | 0.10 | ||
200 | 4.29% | CV < 5% | 1.68 | 0.12 | 1.67 | 0.46 | ||||||
100 | 3.67% | CV < 5% | 2.08 | 0.27 | 1.22 | 0.42 | ||||||
50 | 4.14% | CV < 5% | 2.76 | 0.74 | 1.61 | 0.73 | ||||||
25 | 19.58% | CV < 20% | 1.49 | 0.90 | 0.55 | 0.16 | ||||||
QIAamp DNA Kit | 400 | 9.57% | CV < 10% | <0.01 | 1.49 | 0.02 | 0.48 | 2.18 | 0.88 | 0.25 | ||
200 | 4.85% | CV < 5% | 1.86 | 0.38 | 1.76 | 0.87 | ||||||
100 | 3.15% | CV < 5% | 1.86 | 0.69 | 1.00 | 0.76 | ||||||
50 | 22.17% | CV < 30% | 1.72 | 0.58 | 2.21 | 1.30 | ||||||
25 | 2.99% | CV < 5% | 1.36 | 0.14 | 0.90 | 0.39 | ||||||
miRNeasy Tissue Kit | 400 | 2.68% | CV < 5% | < 0.01 | 2.13 | 0.37 | 0.72 | 0.14 | 0.09 | 0.22 | ||
200 | 7.53% | CV < 10% | 2.15 | 0.25 | 0.05 | 0.04 | ||||||
100 | 19.87% | CV < 20% | 2.10 | 0.42 | 0.04 | 0.02 | ||||||
50 | 4.63% | CV < 5% | 1.89 | 0.31 | 0.04 | 0.02 | ||||||
25 | 3.54% | CV < 5% | 1.85 | 0.41 | 0.080 | 0.04 | ||||||