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Fig. 8 | International Journal of Legal Medicine

Fig. 8

From: Evaluation of extraction methods for co-isolation of nucleic acid from human saliva for forensic body fluid identification

Fig. 8

Sensitivity of nucleic acid extraction methods to changes in sample volume. The x-axis represents the sample volume (25 µL, 50 µL, 100 µL, 200 µL, 400 µL) and the y-axis represents the miRNA yield. Each data point represents the median miRNA yield from three replicate experiments for the given sample volume. The R² value indicates the strength of the relationship between input volume and miRNA yield, reflecting how well each extraction method responds to changes in sample volume (A) A strong, statistically significant linear relationship was observed for the AccuPrep® Genomic DNA Extraction Kit, with 99.80% of the variability in miRNA yield explained by input volume. This indicates consistent miRNA extraction across varying input volumes (B) A non-significant relationship was observed for the QIAamp DNA Mini Kit, with only 62.84% of the variability in miRNA yield explained by input volume, demonstrating inconsistent performance across varying volumes (C) A significant linear relationship was observed for the miRNeasy Tissue/Cells Advanced Micro Kit, with 82.17% of the variability in RNA yield explained by input volume. However, the graph does not strongly emphasize sensitivity to smaller changes in sample input volume, particularly at the lower end (e.g., 100 µL and below)

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