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Fig. 6 | International Journal of Legal Medicine

Fig. 6

From: Evaluation of extraction methods for co-isolation of nucleic acid from human saliva for forensic body fluid identification

Fig. 6

Sensitivity of nucleic acid extraction methods to changes in sample volume. The x-axis represents the sample volume (25 µL, 50 µL, 100 µL, 200 µL, 400 µL) and the y-axis represents the RNA yield. Each data point represents the median RNA yield from three replicate experiments for the given sample volume. The R² value indicates the strength of the relationship between input volume and RNA yield, reflecting how well each extraction method responds to changes in sample volume (A) A strong, statistically significant linear relationship was observed for the AccuPrep® Genomic DNA Extraction Kit, with 99.92% of the variability in RNA yield explained by input volume. This indicates consistent RNA extraction across varying input volumes (B) A statistically significant linear relationship was observed for the QIAamp DNA Mini Kit, with 98.62% of the variability in RNA yield explained by input volume, demonstrating consistent performance across varying volumes (C) A non-significant relationship was observed for the miRNeasy Tissue/Cells Advanced Micro Kit, with only 60.29% of the variability in RNA yield explained by input volume. This suggests inconsistent RNA extraction across varying sample volumes

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