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Fig. 4 | International Journal of Legal Medicine

Fig. 4

From: Evaluation of extraction methods for co-isolation of nucleic acid from human saliva for forensic body fluid identification

Fig. 4

Sensitivity of nucleic acid extraction methods to changes in sample volume. The x-axis represents the sample volume (25 µL, 50 µL, 100 µL, 200 µL, 400 µL) and the y-axis represents the DNA yield. Each data point represents the median DNA yield from three replicate experiments for the given sample volume. The R² value indicates the strength of the relationship between input volume and DNA yield, reflecting how well each extraction method responds to changes in sample volume (A) A stron, statistically significant linear relationship was observed for the Bioneer AccuPrep® Genomic DNA Extraction Kit, with 99.90% of the variability in DNA yield explained by input volume. This indicates consistent DNA extraction across varying input volumes (B) A statistically significant linear relationship was observed for the QIAamp DNA Mini Kit with 90.55% of the variability in DNA yield explained by input volume, demonstrating consistent performance across varying volumes (C) A non-significant relationship was observed for the miRNeasy Tissue/Cells Advanced Micro Kit, with only 77.03% of the variability in DNA yield explained by input volume. This suggests inconsistent DNA extraction across varying sample volumes

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