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Concordance analysis of diatom types and patterns in lung tissue and drowning medium in laboratory animal model

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Abstract

Diatom test has been widely used in the diagnosis of drowning and inferring the drowning site. One of the issues is whether the concordance of the diatom types and patterns between the drowning victim’s organs and media should be considered an essential requirement for the diagnosis of drowning. In this study, lung tissues from 20 rabbits and drowning media were studied by the Microwave Digestion–Vacuum Filtration–Automated Scanning Electron Microscopy method, and four methods, type consistency, coefficient of similarity, squared-chord distance, and cluster analysis, were introduced to analyze the diatom types and patterns for evaluating the value of diatom consistency in drowning cases. The results showed that diatom types and patterns in lung tissues do not perfectly match the drowning medium, and they are sometimes concordant with the drowning medium sampled from other than drowning site. We should be cautious when using diatom detection to infer drowning sites.

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Acknowledgements

The authors gratefully thank Dr Sherry Ma for assistance in preparing the manuscript. This study was financially supported by Guangzhou Municipal Science and Technology Project (2019030001; 2019030012) and Grant-in Aids for Scientific Research from Ministry of Public Security of the People’s Republic of China (2020GABJC38).

Funding

Science and technology commission of shanghai municipality,2019030001,Jian Zhao,guangzhou science,technology and innovation commission,2019030012,Chao Liu,ministry of public security of the people's republic of china,2020GABJC38,Chao Liu

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Correspondence to Chao Liu, Huijun Wang or Jian Zhao.

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Du, Y., Liu, J., Li, Q. et al. Concordance analysis of diatom types and patterns in lung tissue and drowning medium in laboratory animal model. Int J Legal Med 136, 911–917 (2022). https://doi.org/10.1007/s00414-021-02768-9

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  • DOI: https://doi.org/10.1007/s00414-021-02768-9

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