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Automated diatom searching in the digital scanning electron microscopy images of drowning cases using the deep neural networks

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Abstract

Forensic diatom test has been widely accepted as a way of providing supportive evidences in the diagnosis of drowning. The current workflow is primarily based on the observation of diatoms by forensic pathologists under a microscopy, and this process can be very time-consuming. In this paper, we demonstrate a deep learning-based approach for automatically searching diatoms in scanning electron microscopic images. Cross-validation studies were performed to evaluate the influence of magnification on performance. Moreover, various training strategies were tested to improve the performance of detection. The conclusion shows that our approach can satisfy the necessary requirements to be integrated as part of an automatic forensic diatom test.

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Funding

This study was financially supported by Grant-in Aids for Scientific Research from Ministry of Public Security of the People’s Republic of China (2019SSGG0403; 2020SSTG0401), and grant from the Guangzhou Municipal Science and Technology Project (2019030001; 2019030012).

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Correspondence to Chao Liu or Jian Zhao.

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The authors declare that they have no conflict of interest.

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The principles outlined in the Declaration of Helsinki were followed. The project was approved by Guangzhou Forensic Science Institute.

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Informed consent was obtained from the legal representatives of the death cases.

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Yu, W., Xue, Y., Knoops, R. et al. Automated diatom searching in the digital scanning electron microscopy images of drowning cases using the deep neural networks. Int J Legal Med 135, 497–508 (2021). https://doi.org/10.1007/s00414-020-02392-z

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  • DOI: https://doi.org/10.1007/s00414-020-02392-z

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