Skip to main content
SpringerLink
Log in

German population data for 18 X-STRs: a hexaplex PCR adding two clusters of X-STRs to the Argus X-12 set and expanding the German haplotype databases

  • Population Data
  • Published:
International Journal of Legal Medicine Aims and scope Submit manuscript

Abstract

In kinship analysis, large data sets with estimated haplotype frequencies for marker clusters are very important for the likelihood calculation. Practical use of the X-STRs demonstrated that in some complex kinship cases, the marker set of the Investigator Argus X-12 kit can be insufficient. This study aimed to extend the German data base of the Argus X-12 kit (1037 haplotypes) and for a cluster in Xq21 (806 haplotypes) with additional 700 male haplotypes and to include a further cluster in Xp22.3 to complete the X-STR marker set for complex kinship cases.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Szibor R (2007) X-chromosomal markers: past, present and future. Forensic Sci Int Genet 1:93–99. https://doi.org/10.1016/j.fsigen.2007.03.003

  2. Turrina S, Atzei R, Filippini G, De Leo D (2007) Development and forensic validation of a new multiplex PCR assay with 12 X-chromosomal short tandem repeats. Forensic Sci Int Genet 1:201–204. https://doi.org/10.1016/j.fsigen.2014.11.008

  3. Gusmão L et al (2009) A GEP-ISFG collaborative study on the optimization of an X-STR decaplex: data on 15 Iberian and Latin American populations. Int J Legal Med 123:227–234. https://doi.org/10.1007/s00414-008-0309-4

  4. Hering S, Edelmann J, Augustin C, Kuhlisch E, Szibor R (2010) X chromosomal recombination-a family study analysing 39 STR markers in German three-generation pedigrees. Int J Legal Med 124:483–491. https://doi.org/10.1007/s00414-009-0387-y

  5. Poetsch M, Knop A, El-Mostaqim D, Rakotomavo N, von Wurmb-Schwark N (2011) Allele frequencies of 11 X chromosomal STRs of two African populations. Int J Legal Med 125:307–314. https://doi.org/10.1007/s00414-010-0451-7

  6. Diegoli TM, Linacre A, Schanfield MS, Coble MD (2014) Mutation rates of 15 X chromosomal short tandem repeat markers. Int J Legal Med 128:579–587. https://doi.org/10.1007/s00414-014-1016-y

  7. Tillmar AO, Kling D, Butler JM, Parson W, Prinz M, Schneider PM, Egeland T, Gusmão L (2017) DNA Commission of the International Society for Forensic Genetics (ISFG): guidelines on the use of X-STRs in kinship analysis. Forensic Sci Int Genet 29:269–275. https://doi.org/10.1016/j.fsigen.2017.05.005

  8. Kling D, Dell’Amico B, Tillmar AO (2015) FamLinkX - implementation of a general model for likelihood computations for X-chromosomal marker data. Forensic Sci Int Genet 17:1–7. https://doi.org/10.1016/j.fsigen.2015.02.007

  9. Egeland T, Kling D, Mostad P (2016) Relationship inference with familias and R: statistical methods in forensic geneticss. Academic Press, Elsevier Ltd, London

  10. Hering S, Edelmann J, Haas S, Grasern N (2015) Paternity testing of two female siblings with Investigator Argus X-12 kit: a case with several rare mutation and recombination events. Forensic Sci Int Genet Suppl Ser 5:e341–e343

  11. Nishi T, Fukui K, Iwadate K (2017) Analysis of four novel X-chromosomal short tandem repeats within 71 kb of the Xp22.3 region. Int J Legal Med 131(5):1229–1233. https://doi.org/10.1007/s00414-017-1553-2

  12. Szibor R, Hering S, Kuhlisch E, Plate I, Demberger S, Krawczak M, Edelmann J (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369. https://doi.org/10.1007/s00414-005-0550-z

  13. Edelmann J, Lutz-Bonengel S, Naue J, Hering S (2012) X-chromosomal haplotype frequencies of four linkage groups using the Investigator Argus X-12 Kit. Forensic Sci Int: Genet 6(1):e24–e34. https://doi.org/10.1016/j.fsigen.2011.01.001

Download references

Acknowledgments

We thank all sample donors for their contribution to this work.

Author information

Authors and Affiliations

  1. Institute of Legal Medicine, University of Dresden, Dresden, Germany

    Sandra Hering

  2. Institute for Medical Informatics and Biometry, University of Dresden, Dresden, Germany

    Anna Klimova

  3. Institute of Legal Medicine, University of Leipzig, Leipzig, Germany

    Jeanett Edelmann

Authors
  1. Sandra Hering
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anna Klimova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jeanett Edelmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jeanett Edelmann.

Ethics declarations

The study was conducted in compliance with the ethical standards. All the procedures prior to their implementation were approved by the Ethical Committee of the University of Dresden.

Conflict of interest

The authors declare that they have no conflict of interest

Informed consent

All persons with informed consent (vote EK 210052019 of the ethics committee, University of Dresden, Germany)

Quality assurance

The Institute of Legal Medicine is an ISO/IEC 17025; 2005 accredited laboratory and participates regularly in quality assurance exercises.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(XLSX 11 kb)

ESM 2

(XLSX 71 kb)

ESM 3

(XLSX 39 kb)

ESM 4

(XLSX 13 kb)

ESM 5

(XLSX 84 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hering, S., Klimova, A. & Edelmann, J. German population data for 18 X-STRs: a hexaplex PCR adding two clusters of X-STRs to the Argus X-12 set and expanding the German haplotype databases. Int J Legal Med 134, 2061–2062 (2020). https://doi.org/10.1007/s00414-020-02306-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00414-020-02306-z

Keywords

Search

Navigation