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A SNP panel for early detection of artificial chimerism in HSCT patients using TaqMan technology

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Abstract

The monitoring of chimerism status in a hematopoietic stem cell transplantation patient is a crucial process and is performed periodically in a short time interval. A short tandem repeat marker is widely used for chimerism analysis due to its high discrimination power. However, the sensitivity of this approach was limited to 5% of a minor contributor and the interpretation is usually interrupted with PCR stochastic phenomena. Here, we developed an SNP panel for chimerism analysis using TaqMan technology. A set of SNPs was selected from Thai ancestry informative markers and open-access databases with proper criteria. We examined the 30 recipient-donor pairs that underwent HSCT and showed that the panel can provide an informative marker from 90% of all pairs. An early detection of artificial chimerism in post-HSCT samples was observed when compared with STR analysis. In addition, the detail of cases was discussed.

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Acknowledgments

We would like to appreciate our colleagues from the Forensic Genetics Research Unit, Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, who provided insight and expertise that greatly assisted this research.

Funding

This study was supported by the Ratchadapiseksompotch Fund, Faculty of Medicine, Chulalongkorn University.

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Authors and Affiliations

  1. Department of Forensic Medicine, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand

    Tikumphorn Sathirapatya, Wikanda Worrapitirungsi, Poonyapat Sukawutthiya, Kawin Rasmeepaisarn & Kornkiat Vongpaisarnsin

  2. Forensic Serology and DNA, King Chulalongkorn Memorial Hospital and Thai Red Cross Society, Bangkok, Thailand

    Tikumphorn Sathirapatya, Wikanda Worrapitirungsi, Poonyapat Sukawutthiya, Kawin Rasmeepaisarn & Kornkiat Vongpaisarnsin

  3. Forensic Genetics Research Unit, Ratchadapiseksompotch Fund, Faculty of Medicine, Chulalongkorn University, Bangkok, 10300, Thailand

    Kornkiat Vongpaisarnsin

Authors
  1. Tikumphorn Sathirapatya
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  2. Wikanda Worrapitirungsi
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  3. Poonyapat Sukawutthiya
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  4. Kawin Rasmeepaisarn
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  5. Kornkiat Vongpaisarnsin
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Correspondence to Kornkiat Vongpaisarnsin.

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This study was approved by the Institutional Review Board, Faculty of Medicine, Chulalongkorn University (IRB No.705/59).

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The authors declare that they have no conflict of interest.

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Sathirapatya, T., Worrapitirungsi, W., Sukawutthiya, P. et al. A SNP panel for early detection of artificial chimerism in HSCT patients using TaqMan technology. Int J Legal Med 134, 1553–1561 (2020). https://doi.org/10.1007/s00414-020-02276-2

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  • DOI: https://doi.org/10.1007/s00414-020-02276-2

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