Abstract
In the present study, allele frequencies and forensic parameters of four ethnic groups (Brahmin, Khatri, Jat Sikh, and Scheduled Caste) of Punjab, India, at 10 Alu insertions of X chromosome were calculated. Six Alu markers were observed to be highly polymorphic with no significant deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium present in any marker. Multidimensional plot showed higher genetic affinity of studied populations with Asian populations. Overall, the tested markers were reliable and were found suitable in human forensics and population genetic studies.
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References
Schaffner SF (2004) The X chromosome in population genetics. Nat Rev Genet 5(1):43–51
Ahloowalia BS (2009) Invasion of the genes genetic heritage of India. Strategic Book 273 Publishing
Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16(3):1215
Singh G, Talwar I, Sharma R, Sandhu HS, Matharoo K, Bhanwer AJ (2016) Analysis of ANKKI (rs1800497) and DRD2 (rs1079597, rs1800498) variants in five ethnic groups from Punjab, North-West India. Gene 584(1):69–74
Callinan PA, Hedges DJ, Salem AH, Xing J, Walker JA, Garber RK, Watkins WS, Bamshad MJ, Jorde LB, Batzer MA (2003) Comprehensive analysis of Alu-associated diversity on the human sex chromosomes. Gene. 317:103–110
Szibor R, Hering S, Edelmann J (2006) A new web site compiling forensic chromosome X research is now online. Int J Legal Med 120(4):252–254
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics. 155(2):945–959
Singh G, Sandhu HS, Sharma R, Srinivas Y, Matharoo K, Singh M, Bhanwer AJ (2019) Genetic variation and population structure of five ethnic groups from Punjab, North-West India: analysis of MHC class I polymorphic Alu insertions (POALINs). Gene. 701:173–178
Singh G, Talwar I, Sandhu HS, Matharoo K, Bhanwer AJ (2017) Genetic dissection of five ethnic groups from Punjab, North-West India–a study based on autosomal markers. Legal Med 26:25–32
Poetsch M, Bajanowski T, Pfeiffer H (2012) The publication of population genetic data in the international journal of legal medicine: guidelines. Int J Legal Med 126:489–490
Acknowledgments
The authors are grateful to all of the donors for providing blood samples and to the people who contributed to the collection. They are especially thankful to Dr. Jim Lemon for helping with R codes needed for the genetic analysis.
Funding
This study is financially supported by the Department of Biotechnology (DBT), Government of India, through project grant BT/PR2722/Med/13/121/2001 (DBT, India) to Bhanwer AJS at Guru Nanak Dev University Amritsar.
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Singh, G., Sandhu, H.S., Srinivas, Y. et al. Genetic portrait of North-West Indian population based on X chromosome Alu insertion markers. Int J Legal Med 134, 1655–1657 (2020). https://doi.org/10.1007/s00414-019-02238-3
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DOI: https://doi.org/10.1007/s00414-019-02238-3