Abstract
Genetic characterization of ethnically and geographically diverse populations via short tandem repeats (STRs) is relevant to various fundamental and applied areas of forensic genetics, population studies, and even molecular anthropology. In the present study, genetic polymorphisms of 15 autosomal STR loci were firstly obtained from 918 individuals (495 Nakhis and 423 Yis) residing in the foothills of the Himalayas. The cumulative powers of discrimination and probabilities of exclusion in the two studied ethnic groups were both larger than 0.999999999999999982 and 0.9999961, respectively. Genetic similarities and differences among 61 populations were subsequently investigated by pairwise Cavalli-Sforza genetic distance, multidimensional scaling plots, principal component analysis, and phylogenetic relationship reconstruction. Both Nakhi and Yi had the genetically close relationships with Yunnan Bai and distinct relationships with Xinjiang Turkic-speaking populations (Uyghur and Kazakh) and Vietnamese.
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Gymrek M, Willems T, Reich D, Erlich Y (2017) Interpreting short tandem repeat variations in humans using mutational constraint. Nat Genet 49:1495–1501. https://doi.org/10.1038/ng.3952
Gymrek M (2017) A genomic view of short tandem repeats. Curr Opin Genet Dev 44:9–16. https://doi.org/10.1016/j.gde.2017.01.012
He G, Wang Z, Wang M, Hou Y (2017) Genetic diversity and phylogenetic differentiation of southwestern Chinese Han: a comprehensive and comparative analysis on 21 non-CODIS STRs. Sci Rep 7:13730. https://doi.org/10.1038/s41598-017-13190-w
He G, Chen P, Zou X, Chen X, Song F, Yan J, Hou YP (2017) Genetic polymorphism investigation of the Chinese Yi minority using PowerPlex(R) Y23 STR amplification system. Int J Legal Med 131:663–666. https://doi.org/10.1007/s00414-017-1537-2
He G, Li Y, Zou X, Li P, Chen PY, Song F, Gao T, Liao M, Yan J, Wu J (2017) Forensic characteristics and phylogenetic analyses of the Chinese Yi population via 19 X-chromosomal STR loci. Int J Legal Med 131:1243–1246. https://doi.org/10.1007/s00414-017-1563-0
He G, Wang Z, Zou X, Chen X, Liu J, Wang M, Hou Y (2018) Genetic diversity and phylogenetic characteristics of Chinese Tibetan and Yi minority ethnic groups revealed by non-CODIS STR markers. Sci Rep 8:5895. https://doi.org/10.1038/s41598-018-24291-5
Walsh PS, Metzger DA, Higuchi R (1991) Chelex-100 as a medium for simple extraction of DNA for Pcr-based typing from forensic material. BioTechniques 10:506–513
Poetsch M, Bajanowski T, Pfeiffer H (2012) The publication of population genetic data in the International Journal of Legal Medicine: guidelines. Int J Legal Med 126:489–490. https://doi.org/10.1007/s00414-012-0700-z
Gouy A, Zieger M (2017) STRAF-A convenient online tool for STR data evaluation in forensic genetics. Forensic Sci Int Genet 30:148–151. https://doi.org/10.1016/j.fsigen.2017.07.007
Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054
Kovach WL (2007) MVSP-A MultiVariate Statistical Package for Windows, version 3.1. Kovach Computing Services, Pentraeth, Wales, UK
Funding
This study received the support of grants from the National Key R&D Program of China (2016YFC0800703), the National Natural Science Foundation of China (81571854 and 81501635), and the Fundamental Research Funds for the Central Universities (20826041A4408, YJ201651, and 2012017yjsy187).
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Human blood samples were collected with the approval of the Ethics Committee of Sichuan University (Approval Number: K2015008).
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The authors declare that they have no competing interests.
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He, G., Su, Y., Zou, X. et al. Allele frequencies of 15 autosomal STRs in Chinese Nakhi and Yi populations. Int J Legal Med 133, 105–108 (2019). https://doi.org/10.1007/s00414-018-1931-4
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DOI: https://doi.org/10.1007/s00414-018-1931-4