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Genetic variation of 17 X-chromosome STR loci in Tunisian population of Nabeul

  • Monia Messoussi
  • Endika Prieto-Fernández
  • Miriam Baeta
  • Carolina Núñez
  • Amel Ben Ammar-El Gaaied
  • Marian M. de Pancorbo
  • Karima Fadhlaoui-Zid
Population Data

Abstract

In the present study, the genetic variations of 17 X-STR markers (DXS8378, DXS9898, DXS7133, GATA31E08, GATA172D05, DXS6801, DXS7423, DXS6809, DXS6799, DXS7132, DXS9902, DXS6800, DXS6789, DXS10075, DXS10079, DXS6807, and DXS6803) were analyzed in 139 unrelated individuals in Nabeul, aiming to perform an X-STR database for anthropological and forensic purposes. Our results indicate that DXS6809 was the most polymorphic locus, whereas DXS6807 was the least informative marker. In addition, the obtained values for the statistical parameters of forensic interest, i.e., the power of discrimination in males (PDM) and females (PDF), as well as the mean exclusion chance in duos (MECD) and trios (MECT) have demonstrated that this panel of 17 X-STRs is highly informative and useful for forensic application and anthropological research. Additionally, pairwise genetic distances based on FST were calculated between Nabeul population and other populations extracted from the literature. Genetic distances were represented in a non-metric MDS plot and clustering of populations according to their geographic locations and their historical relationship was detected.

Keywords

Nabeul city Tunisia X-chromosome database Short tandem repeat Forensic application 

Notes

Acknowledgements

The authors are grateful to Maite Alvarez, PhD for her technical and human support provided by the DNA Bank Service (SGIker) of the University of the Basque Country (UPV/EHU) and European funding (ERDF and ESF). The authors also thank Dr. Asmahan Bekada for providing X-STR data.

Funding information

Funds were provided by the Basque Government (Grupo Consolidado IT998-16) and the Tunisian Ministry of Higher Education and Scientific Research.

Compliance with ethical standards

All samples were obtained from volunteer donors under informed consent, following the ethical standards of Helsinki Declaration. The current study was approved by the local Ethics Committee of Charles Nicolle Hospital in Tunis.

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Monia Messoussi
    • 1
  • Endika Prieto-Fernández
    • 2
  • Miriam Baeta
    • 2
  • Carolina Núñez
    • 2
  • Amel Ben Ammar-El Gaaied
    • 1
  • Marian M. de Pancorbo
    • 2
  • Karima Fadhlaoui-Zid
    • 1
  1. 1.Laboratory of Genetics, Immunology, and Human Pathologies, Faculty of Science of TunisUniversity Tunis El ManarTunisTunisia
  2. 2.BIOMICs Research Group, Lascaray Research CenterUniversity of the Basque Country UPV/EHUVitoria-GasteizSpain

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