The decomposition process of human remains can be used to estimate the post-mortem interval (PMI), but decomposition varies due to many factors. Temperature is believed to be the most important and can be connected to decomposition by using the accumulated degree days (ADD). The aim of this research was to develop a decomposition scoring method and to develop a formula to estimate the PMI by using the developed decomposition scoring method and ADD.
A decomposition scoring method and a Book of Reference (visual resource) were made. Ninety-one cases were used to develop a method to estimate the PMI. The photographs were scored using the decomposition scoring method. The temperature data was provided by the Royal Netherlands Meteorological Institute. The PMI was estimated using the total decomposition score (TDS) and using the TDS and ADD. The latter required an additional step, namely to calculate the ADD from the finding date back until the predicted day of death.
The developed decomposition scoring method had a high interrater reliability. The TDS significantly estimates the PMI (R 2 = 0.67 and 0.80 for indoor and outdoor bodies, respectively). When using the ADD, the R 2 decreased to 0.66 and 0.56.
The developed decomposition scoring method is a practical method to measure decomposition for human remains found on land. The PMI can be estimated using this method, but caution is advised in cases with a long PMI. The ADD does not account for all the heat present in a decomposing remain and is therefore a possible bias.
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Cockle DL, Bell LS (2015) Human decomposition and the reliability of a ‘Universal’ model for post mortem interval estimations. Forensic Sci Int 253:136:e1–136:e9. https://doi.org/10.1016/j.forsciint.2015.05.018
Henßge C, Madea B (2004) Estimation of the time since death in the early post-mortem period. Forensic Sci Int 144:167–175. https://doi.org/10.1016/j.forsciint.2004.04.051
Warther S, Sehner S, Raupach T, Püschel K, Anders S (2012) Estimation of the time since death: post-mortem contractions of human skeletal muscles following mechanical stimulation (idiomuscular contraction). Int J Legal Med 126:399–405. https://doi.org/10.1007/s00414-011-0665-3
Madea B (2016) Supravitality in tissues. In: Madea B (ed) Estimation of the time since death. CRC Press LLC, Boca Raton, pp 17–40
Megyesi MS, Nawrocki SP, Haskell NH (2005) Using accumulated degree-days to estimate the postmortem interval from decomposed human remains. J Forensic Sci 50(3):618–626
Sutherland A, Myburgh J, Steyn M, Becker PJ (2013) The effect of body size on the rate of decomposition in a temperate region of South Africa. Forensic Sci Int 231(1–3):257–262. https://doi.org/10.1016/j.forsciint.2013.05.035
Matuszewski S, Konwerski S, Fratczak K, Szafalowicz M (2014) Effect of body mass and clothing on decomposition of pig carcasses. Int J Legal Med 128:1039–1048. https://doi.org/10.1007/s00414-014-0965-5
Suckling JK, Spradley MK, Godde K (2015) A longitudinal study on human outdoor decomposition in Central Texas. J Forensic Sci 61:19–25. https://doi.org/10.1111/1556-4029.12892
Johnson AP, Mikac KM, Wallman JF (2013) Thermogenesis in decomposing carcasses. Forensic Sci Int 231:271–277 https://doi.org/10.1016/j.forsciint.2013.05.031
Campobasso CP, Di Vella G, Introna F (2001) Factors affecting decomposition and Diptera colonization. Forensic Sci Int 120:18–27. https://doi.org/10.1016/S0379-0738(01)00411-X
Mann RW, Bass WM, Meadows L (1990) Time since death and decomposition of the human body: variable and observations in case and experimental field studies. J Forensic Sci 35(1):103–111. https://doi.org/10.1520/JFS12806J
Damann FE, Carter DO (2014) Human decomposition ecology and postmortem microbiology. In: Pokines JT, Symes SA (eds) Manual of forensic taphonomy. CRC Press LLC, Boca Raton, pp 37–49
Bass WM (1996) Outdoor decomposition rates in Tennessee. In: Haglund WD, Sorg MH (eds) Forensic taphonomy: the postmortem fate of human remains. CRC Press LLC, Boca Raton, pp 181–186
Simmons T, Adlam RE, Moffatt C (2010) Debugging decomposition data—comparative taphonomic studies and the influence of insects and carcass size on decomposition rate. J Forensic Sci 50(1):8–13. https://doi.org/10.1111/j.1556-4029.2009.01206.x
Payne JA (1965) A summer carrion study of the baby pig Sus scrofa Linnaeus. Ecology 45(5):592–602. http://dx.doi.org/10.2307/1934999
Anderson GS (2000) Minimum and maximum development rates of some forensically important Calliphoridae (Diptera). J Forensic Sci 45(4):824–832. https://doi.org/10.1520/JFS14778J
Dautartas AM (2009) The effect of various coverings on the rate of human decomposition. Master’s Thesis, University of Tennessee
Zhou C, Byard RW (2011) Factors and processes causing accelerated decomposition in human cadavers—an overview. J Forensic Leg Me 18:6–9. https://doi.org/10.1016/j.jflm.2010.10.003
Spicka A, Johnson R, Bushing J, Higley LG, Carter DO (2011) Carcass mass can influence rate of decomposition and release of ninhydrin-reactive nitrogen into gravesoil. Forensic Sci Int 209:80–85. https://doi.org/10.1016/j.forsciint.2011.01.002
Kelly JA, van der Linde TC, Anderson GS (2009) The influence of clothing and wrapping on carcass decomposition and arthropod succession during the warmer seasons in Central South Africa. J Forensic Sci 54(5):1105–1112. https://doi.org/10.1111/j.1556-4029.2009.01113.x
Voss SC, Cook DF, Dadour IR (2011) Decomposition and insect succession of clothed and unclothed carcasses in Western Australia. Forensic Sci Int 211:67–75. https://doi.org/10.1016/j.forsciint.2011.04.018
Vass AA, Bass WM, Wolt JD, Foss JE, Ammons JT (1992) Time since death determinations of human cadavers using soil solution. J Forensic Sci 37(5):1236–1253. https://doi.org/10.1520/JFS13311J
Payne-James J, Jones R, Karch SB, Manlove J (2011) Simpson’s forensic medicine, 13th edn. Hodder Arnold, London
Galloway A, Birkby WH, Jones AM, Henry TE, Parks BO (1989) Decay rates of human remains in an arid environment. J Forensic Sci 34(3):607–616. https://doi.org/10.1520/JFS12680J
Anderson GS, VanLaerhoven SL (1996) Initial studies on insect succession on carrion in southwestern British Columbia. J Forensic Sci 41(4):617–625. https://doi.org/10.1520/JFS13964J
Shalaby OA, deCarvalho LML, Goff ML (2000) Comparison of patterns of decomposition in a hanging carcass and a carcass in contact with soil in a xerophytic habitat on the Island of Oahu, Hawaii. J Forensic Sci 45(6):1267–1273. https://doi.org/10.1520/JFS14877J
Dabbs GR, Connor M, Bytheway JA (2016) Interobserver reliability of the total body score system for quantifying human decomposition. J Forensic Sci 61(2):445–451. http://dx.doi.org/10.1111/1556-4029.12962
Nawrocka M, Fratczak K, Matuszewski S (2016) Inter-rater reliability of total body score—a scale for quantification of corpse decomposition. J Forensic Sci 61(3):798–802. https://doi.org/10.1111/1556-4029.13105
Randolph JJ. Online Kappa Calculator. http://justus.randolph.name/kappa; 2008 Access 16.01.26
Rice PL, Orgill DP. Classification of burns. https://www.uptodate.com.proxy-ub.rug.nl/contents/classification-of-burns?source=search_result&search=rule+of+nine&selectedTitle=1~5#H16; 2016 Access 16.03.21
Bijl D, Semmekrot B, van Loenen A (2013) Farmacotherapie. In: Bindels PJE, Kneepkens CMF (eds) Kindergeneeskunde. Bohn Stafleu van Loghum, Houten, pp 85–86
van Daalen MA, de Kat DS, Oude Grotebevelsborg BFL, Warnaar J, Oostra RJ, Duijst-Heesters WLJM (2017) An aquatic decomposition scoring method to potentially predict the postmortem submersion interval of bodies recovered from the North Sea. J Forensic Sci 62(2):369–373. https://doi.org/10.1111/1556-4029.13258
Rodriguez WC (1996) Decomposition of buried and submerged bodies. In: Haglund WD, Sorg MH (eds) Forensic taphonomy: the postmortem fate of human remains. CRC Press LLC, Boca Raton, pp 459–467
Vass AA (2011) The elusive universal post-mortem interval formula. Forensic Sci Int 204:34–40. https://doi.org/10.1016/j.forsciint.2010.04.052
Koninklijk Nederlands Meteorologisch Instituut, Klimatologie—Daggegevens van het weer in Nederland. http://projects.knmi.nl/klimatologie/daggegevens/selectie.cgi; Accessed 15.12.12 until 16.02.22
Micozzi MS (1996) Frozen environments and soft tissue preservation. In: Haglund WD, Sorg MH (eds) Forensic taphonomy: the postmortem fate of human remains. CRC Press LLC, Boca Raton, pp 171–180
Madea B, Henssge C, Reibe S, Tsokos M, Kernbach-Wighton G (2014) Postmortem changes and time since death. In: Madea B (ed) Handbook of forensic medicine. John Wiley & Sons, West Sussex, pp 75–133
Knight B, Saukko P (2004) Knight’s forensic pathology. Hodder Arnold, London
Lee Goff M (2009) Early post-mortem changes and stages of decomposition in exposed cadavers. Exp Appl Acarol 49:21–36. https://doi.org/10.1007/s10493-009-9284-9
Reijnders UJL, Das C (2007) De lijkschouw in de praktijk. Prelum Uitgevers, Maarn
Micozzi MS (1991) Postmortem change in human and animal remains. Charles C Thomas, Springfield
Bucholtz A (2015) Death investigation. Anderson Publishing, Waltham
Van Voorde W (2016) Forensische Geneeskunde, 3rd ed. die Keure, Brugge
Clark MA, Worrell MB, Pless JE (1996) Postmortem changes in soft tissues. In: Haglund WD, Sorg MH (eds) Forensic taphonomy: the postmortem fate of human remains. CRC Press LLC, Boca Raton, pp 151–160
Marais-Werner A (2016) Decomposition patterns of buried remains in the central Highveld region of South Africa. Dissertation, University of Pretoria
Afstand berekenen. https://nl.afstand.org/. Accessed 17.01.30
Statstutor. Spearman’s correlation. https://www.statstutor.ac.uk/resources/uploaded/spearmans.pdf. Accessed 16.02.24
Weatherbase. Flagstaff, Arizona. https://www.weatherbase.com/weather/weather-summary.php3?s=557327&cityname=Flagstaff%2C+Arizona%2C+United+States+of+America&units. Accessed 17.01.29
Visit Arizona. Arizona Weather. https://www.visitarizona.com/arizona-travel-info/arizona-weather. Accessed 17.01.29
Peel MC, Finlayson BL, McMahon TA (2007) Updated world map of the Köppen-Geiger climate classification. Hydrol Earth Syst Sci Discuss 4:439–473. https://doi.org/10.5194/hess-11-1633-2007
Hayman J, Oxenham M (2016) Human body decomposition. Academic Press, London
Bonacci T, Brandmayr P, Greco S, Tersaruolo C, Vercillo V, Brandmayr TZ (2010) A preliminary investigation of insect succession on carrion in Calabria (southern Italy). Terr Arthropod Rev 3:97–110. https://doi.org/10.1163/187498310X517123
Moffatt C, Simmons T, Lynch-Aird J (2016) An improved equation for TBS and ADD: establishing a reliable postmortem interval framework for casework and experimental studies. J Forensic Sci 61(S1):201–207. https://doi.org/10.1111/1556-4029.12931
Villet MH, Richards CS, Midgley JM (2010) Contemporary precision, bias and accuracy of minimum post-mortem intervals estimated using development of carrion-feeding insects. In: Amendt J, Campobasso CP, Goff ML, Grassberger M (eds) Current concepts in forensic entomology. Springer, Dordrecht, pp 109–137
Anderson GS (2001) Insect succession on carrion and its relationship to determining time of death. In: Byrd JH, Castner JL (eds) Forensic entomology: the utility of arthropods in legal investigations. CRC Press LLC, Boca Raton, pp 143–175
This research could not have been conducted without the help of the forensic physicians, forensic scientists and medical students scoring the photographs. We also want to thank the PHS IJsselland and PHS Drenthe. Special thanks to Drs S.P.H. Letmaath. Finally, we want to thank Miss J. van Amerongen for her statistical assistance and Miss J. Ouwejan for providing language help.
This study was approved by the PHS IJsselland and performed according to the ethical and legal standards in the Netherlands. All data were processed anonymously. For this type of study, formal consent is not required.
Conflicts of interest
The authors declare that they have no conflict of interest.
-The development of a decomposition scoring method which has proven to be valid.
-Using the developed and validated decomposition scoring method, the post-mortem interval can be predicted for cases indoors and outdoors with respectively 67 and 80%.
-Accumulated degree days do not seem to control for every heat unit present during the decomposition process.
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Gelderman, H.T., Boer, L., Naujocks, T. et al. The development of a post-mortem interval estimation for human remains found on land in the Netherlands. Int J Legal Med 132, 863–873 (2018). https://doi.org/10.1007/s00414-017-1700-9
- Forensic science
- Forensic taphonomy
- Post-mortem interval
- Decomposition process
- Decomposition phenomena
- Accumulated degree days