Abstract
The X-chromosomal short tandem repeats (STRs) with more informative than autosomal STRs in some complicated biological relationships identification due to its specific mode of genetic transmission can be used as a complementary tool in forensic case practices. In this study, we presented the population genetic data of 19 X-STRs, consisting of DXS10174, DXS10075, DXS10079, DXS101, DXS10101, DXS10103, DXS10134, DXS10135, DXS10148, DXS10159, DXS10162, DXS10164, DXS6789, DXS6809, DXS7132, DXS7423, DXS7424, DXS8378, and HPRTB loci, in a sample of 235 individuals of Tibetan nationality from Sichuan province, Southwest China. All 19 X-STR loci were consistent with Hardy-Weinberg equilibrium. The results showed that the combined power of discrimination in females and males are 0.999999999999999999997 and 0.9999999999997, respectively. In addition, the mean paternity exclusion chances based on the formula of MECKrüger, MECKishida, and MECDesmarais as well as MECDesmarais Duo are 0.99999991, 0.9999999999924, 0.9999999999929, and 0.999999985, respectively. In summary, our findings suggested that the AGCU X19 kit can be considered to serve as a high polymorphic information tool for forensic identification and kinship testing in the Sichuan Tibetan population. Furthermore, population genetic structure investigation between Sichuan Tibetan population and other 19 populations using PCA, MDS, and phylogenetic tree illustrated that significant genetic difference was observed between the Sichuan Tibetan and Malay, as well as the Xinjiang Uyghur population.
Similar content being viewed by others
References
Szibor R (2007) X-chromosomal markers: past, present and future. Forensic Sci Int Genet 1:93–99. https://doi.org/10.1016/j.fsigen.2007.03.003
Samejima M, Nakamura Y, Minaguchi K (2011) Population genetic study of six closely linked groups of X-STRs in a Japanese population. Int J Legal Med 125:895–900. https://doi.org/10.1007/s00414-011-0618-x
Samejima M, Nakamura Y, Nambiar P, Minaguchi K (2012) Genetic study of 12 X-STRs in Malay population living in and around Kuala Lumpur using investigator Argus X-12 kit. Int J Legal Med 126:677–683. https://doi.org/10.1007/s00414-012-0705-7
Deng C, Song F, Li J et al (2017) Forensic parameters of 19 X-STR polymorphisms in two Chinese populations. Int J Legal Med 131:975–977. https://doi.org/10.1007/s00414-017-1538-1
He G, Li Y, Zou X et al (2017) Forensic characteristics and phylogenetic analyses of the Chinese Yi population via 19 X-chromosomal STR loci. Int J Legal Med. https://doi.org/10.1007/s00414-017-1563-0
Yao HB, Wang CC, Wang J et al (2017) Genetic structure of Tibetan populations in Gansu revealed by forensic STR loci. Sci Rep 7:41195. https://doi.org/10.1038/srep41195
Li C, Ma T, Zhao S et al (2011) Development of 11 X-STR loci typing system and genetic analysis in Tibetan and Northern Han populations from China. Int J Legal Med 125:753–756. https://doi.org/10.1007/s00414-011-0592-3
Tillmar AO, Kling D, Butler JM et al (2017) DNA Commission of the International Society for Forensic Genetics (ISFG): guidelines on the use of X-STRs in kinship analysis. Forensic Sci Int Genet 29:269–275. https://doi.org/10.1016/j.fsigen.2017.05.005
Jeong C, Alkorta-Aranburu G, Basnyat B et al (2014) Admixture facilitates genetic adaptations to high altitude in Tibet. Nat Commun 5:3281. https://doi.org/10.1038/ncomms4281
Lorenzo FR, Huff C, Myllymaki M et al (2014) A genetic mechanism for Tibetan high-altitude adaptation. Nat Genet 46:951–956. https://doi.org/10.1038/ng.3067
Racimo F, Sankararaman S, Nielsen R, Huerta-Sanchez E (2015) Evidence for archaic adaptive introgression in humans. Nat Rev Genet 16:359–371. https://doi.org/10.1038/nrg3936
Huerta-Sanchez E, Jin X, Asan et al (2014) Altitude adaptation in Tibetans caused by introgression of Denisovan-like DNA. Nature 512:194–197. https://doi.org/10.1038/nature13408
Matisoff JA (1991) Sino-Tibetan linguistics: present state and future-prospects. Annu Rev Anthropol 20:469–504
Wen B, Xie X, Gao S et al (2004) Analyses of genetic structure of Tibeto-Burman populations reveals sex-biased admixture in southern Tibeto-Burmans. Am J Hum Genet 74:856–865. https://doi.org/10.1086/386292
Walsh PSMD, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10(4):506
Poetsch M, Bajanowski T, Pfeiffer H (2012) The publication of population genetic data in the International Journal of Legal Medicine: guidelines. Int J Legal Med 126:489–490. https://doi.org/10.1007/s00414-012-0700-z
Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10(3):564–567
He G, Chen P, Zou X et al (2017) Genetic polymorphism investigation of the Chinese Yi minority using PowerPlex(R) Y23 STR amplification system. Int J Legal Med 131:663–666. https://doi.org/10.1007/s00414-017-1537-2
Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129
Kumar SSG, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Kovach WL (2007) MVSP-A MultiVariate Statistical Package for Windows, ver. 3.1. Kovach Computing Services, Pentraeth, Wales
Edelmann J, Hering S, Kuhlisch E, Szibor R (2002) Validation of the STR DXS7424 and the linkage situation on the X-chromosome. Forensic Sci Int 125:217–222
Szibor R, Hering S, Kuhlisch E et al (2005) Haplotyping of STR cluster DXS6801-DXS6809-DXS6789 on Xq21 provides a powerful tool for kinship testing. Int J Legal Med 119:363–369. https://doi.org/10.1007/s00414-005-0550-z
Edelmann J, Hering S, Augustin C, Kalis S, Szibor R (2010) Validation of six closely linked STRs located in the chromosome X centromere region. Int J Legal Med 124:83–87. https://doi.org/10.1007/s00414-009-0328-9
Hundertmark T, Hering S, Edelmann J, Augustin C, Plate I, Szibor R (2008) The STR cluster DXS10148-DXS8378-DXS10135 provides a powerful tool for X-chromosomal haplotyping at Xp22. Int J Legal Med 122:489–492
Laan M, VW E, Khusnutdinova M, Remm S (2005) Pääbo. (2005) X-chromosome as a marker for population history: linkage disequilibrium and haplotype study in Eurasian populations. Eur J Hum Genet 13:452–462
He G-L, Chen P-Y, Gao B et al (2017) Phylogenetic analysis among 27 Chinese populations and genetic polymorphisms of 20 autosomal STR loci in a Chinese Uyghur ethnic minority group. Aust J Forensic Sci. https://doi.org/10.1080/00450618.2016.1275793
He G, Li Y, Wang Z et al (2017) Genetic diversity of 21 autosomal STR loci in the Han population from Sichuan province, Southwest China. Forensic Sci Int Genet. https://doi.org/10.1016/j.fsigen.2017.07.006
He G, Wang M, Liu J, Hou Y, Wang Z (2017) Forensic features and phylogenetic analyses of Sichuan Han population via 23 autosomal STR loci included in the Huaxia Platinum System. Int J Legal Med
Acknowledgements
The study was supported by the Sichuan Science and Technology Support Program (2014SZ0012).
Author information
Authors and Affiliations
Contributions
G.H. and Y.L. wrote the manuscript, X.Z. and Y.Z. collected the samples, G.H., Y.L., X.Z., M. W. and H.L. conducted the experiment and analyzed the results, X.Z. modified the manuscript. J.W. conceived the experiment.
Corresponding author
Ethics declarations
Our experiments were approved by the Ethics Committee of Sichuan University, China.
Conflict of interest
The authors declare that they have no conflict of interests.
Electronic supplementary material
Supplementary Fig. 1
(DOCX 290 kb).
Supplementary Fig. 2
(DOCX 91 kb).
Supplementary Fig. 3
(DOCX 191 kb).
Supplementary Fig. 4
(DOCX 31 kb).
Supplementary Fig. 5
(DOCX 155 kb).
Supplementary Table 1
(XLSX 14 kb).
Supplementary Table 2
(XLSX 15 kb).
Supplementary Table 3
(XLSX 11 kb).
Supplementary Table 4
(XLSX 19 kb).
Supplementary Table 5
(XLSX 9 kb).
Supplementary Table 6
(XLSX 13 kb).
Supplementary Table 7
(XLSX 13 kb).
Rights and permissions
About this article
Cite this article
He, G., Li, Y., Zou, X. et al. X-chromosomal STR-based genetic structure of Sichuan Tibetan minority ethnicity group and its relationships to various groups. Int J Legal Med 132, 409–413 (2018). https://doi.org/10.1007/s00414-017-1672-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00414-017-1672-9