Abstract
Application of X-STRs as complements of autosomal STR application in the forensic genetics has become a tendency for kinship testing, especially in deficiency paternity cases. Recently, a novel kit of 19 X-STR loci was developed, which permitted the analysis of 19 STR in the same PCR reaction, and these markers can be clustered into seven groups for the physical linkage. The objective of this study was to evaluate the allele and haplotype diversity of 19 X-STR loci in the Uygur (n = 220) and Tibetan nationality (n = 270) and to estimate the usefulness for complex kinship analysis. In the Tibetan and Uygur populations, a total of alleles of all loci were 188 and 212, with the allele frequencies ranged from 0.0037 to 0.5593 and from 0.0045 to 0.5409, respectively. Compared with previous studies, DXS10135 was the most polymorphic locus in the two population groups, whereas the least variant locus was DXS10164 in the Uygur population and DXS7423 in the Tibetan nationality. Haplotype diversity obtained in this investigation was greater than 0.9 across all LGs. This study indicated the new kit could be used as a supplementary tool in kinship testing in China. In addition, the data sets can be used as supplementary national X-STR references to enlarge the database.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (No. 81302623) and Outstanding Youth Fund of Sichuan University (No. 2016SCU04A07), and the authors would like to thank all contributors who supplied samples to the study.
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The study procedures were approved by the Ethics Committee of Sichuan University, China.
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Chuncao Deng and Feng Song contributed equally to this work.
Feng Song should be considered as co-first author.
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Deng, C., Song, F., Li, J. et al. Forensic parameters of 19 X-STR polymorphisms in two Chinese populations. Int J Legal Med 131, 975–977 (2017). https://doi.org/10.1007/s00414-017-1538-1
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DOI: https://doi.org/10.1007/s00414-017-1538-1