Abstract
Warping has been used to determine the pre-burning condition of human skeletal remains. In the literature, this modification has been associated more often with the burning of fleshed and green bones, but it also arises during the burning of dry bones. The objective of this paper was to assess if bone collagen content has a significant effect on the occurrence of warping in a sample of experimentally burned human bones. The presence of collagen was analyzed in two different samples through a vibrational spectroscopy technology—FTIR. One of them was composed of 40 archeological bones from the seventeenth to twentieth centuries ad. The other one was composed of bones from 14 skeletons belonging to the 21st century identified skeletal collection. The results confirmed that the amide I band assigned to the collagen was much more intense in bones presenting heat-induced warping. Nonetheless, although significant (p = 0.040), the collagen content was not as useful as other variables to the regression model we proposed for explaining warping. Factors such as the maximum temperature (p < 0.001) and burning time (p = 0.001) contributed more significantly. Results demonstrated that the mere preservation of collagen is not enough to explain warping. Burning dynamics seem to have an important role as well although we failed to clearly document its specificities. Other factors such as the asymmetric distribution of collagen and other components within bone, the gravity force, the shape of the bone, and the position in which it is burned may also play an important role on heat-induced changes and require further analysis.
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References
Schmidt CW, Symes SA (eds) (2008) The analysis of burned human remains. Academic, Amsterdam
Symes S, Rainwater C, Chapman E, Gipson DR, Piper A (2008) Patterned thermal destruction of human remains in a forensic setting. In: Schmidt C, Symes S (eds) The analysis of burned human remains. Academic, London, pp 15–54
Ubelaker DH, Rife JL (2007) The practice of cremation in the Roman-era cemetery at Kenchreai, Greece: the perspective from archeology and forensic science. Bioarchaeol Near East 1:35–57
Lemmers SAM (2012) Burned culture: osteological research into Urnfi eld cremation technology and ritual in the South of the Netherlands, Lunula. Archaeol Protohist 20:81–88
Harvig L, Kveiborg J, Lynnerup N (2013) Death in flames: human remains from a domestic house fire from early iron age, Denmark. Int J Osteoarchaeol
Cavazzuti C, Salvadei L (2014) I resti umani cremati dalla necropoli di Casinalbo. In: Cardarelli A (ed) La necropoli della terramara di Casinalbo. All’Insegna del Giglio, Firenze, pp 677–715
Silva FC (2015) The funerary practice of cremation at Augusta Emerita (Mérida, Spain) during High Empire: contributions from the anthropological analysis of burned human bone. In: Thompson TJU (ed) The archaeology of cremation: burned human remains in funerary studies. Oxbow Books, Oxford, pp 123–150
Ulguim P (2015) Analysing cremated human remains from the southern Brazilian highlands: interpreting archaeological evidence of funerary practice at mound and enclosure complexes in the Pelotas River Valley. In: Thompson TJU (ed) The archaeology of cremation: burned human remains in funerary studies. Oxbow Books, Oxford, pp 173–212
Symes SA, L’Abbé EN, Pokines JT, Yuzwa T, Messer D, Stromquist A, Keough N (2014) Thermal alteration to bone. In: Pokines JT, Symes SA (eds) Manual of forensic taphonomy. CRC Press, Boca Raton, pp 367–402
Duday H, Guillon M (2006) Understanding the circumstances of decomposition when the body is skeletonized. In: Schmitt A, Cunha E, Pinheiro J (eds) Forensic anthropology and medicine. Humana Press, Totowa, pp 117–158
Gonçalves D, Cunha E, Thompson TJU (2015) Estimation of the pre-burning condition of human remains in forensic contexts. Int J Legal Med. doi:10.1007/s00414-014-1027-8
Spennemann DHR, Colley SM (1989) Fire in a pit: the effects of burning on faunal remains. Archaeozoologia 3:51–64
Whyte T (2001) Distinguishing remains of human cremations from burned animal bones. J Field Archaeol 28(3/4):437–448
Binford LR (1963) An analysis of cremations from three Michigan sites. Wis Archaeol 44:98–110
Thompson TJU (2005) Heat-induced dimensional changes in bone and their consequences for forensic anthropology. J Forensic Sci 50(5):185–193
Gonçalves D, Thompson TJU, Cunha E (2011) Implications of heat-induced changes in bone on the interpretation of funerary behaviour and practice. J Archaeol Sci 38:1308–1313. doi:10.1016/j.jas.2011.01.006
Zioupos P, Currey JD, Hamer AJ (1999) The role of collagen in the declining mechanical properties of aging human cortical bone. J Biomed Mater Res 45(2):108–116
Very JM, Gibert R, Guilhot B, Debout M, Alexandre C (1997) Effect of aging on the amide group of bone matrix, measured by FTIR spectrophotometry, in adult subjects deceased as a result of violent death. Calcified Tissue Int 60:271–275
Suda HK, Kajiwara M, Matsumoto N, Murayama H, Yamato H (2009) Characterization of apatite and collagen in bone with FTIR imaging. Mol Cryst Liq Cryst 505(1):64/[302]–69/[307]. doi:10.1080/15421400902942144
Bell LS, Skinner MF, Jones SJ (1996) The speed of post mortem change to the human skeleton and its taphonomic significance. Forensic Sci Int 82:129–140
Boaks A, Siwek D, Mortazavi F (2014) The temporal degradation of bone collagen: a histochemical approach. Forensic Sci Int. doi:10.1016/j.forsciint.2014.04.008
Collins MJ, Nielsen-Marsh CM, Hiller J, Smith CI, Roberts JP, Prigodich RV, Wess TJ, Csàpo J, Millard AR, Turner-Walker G (2002) The survival of organic matter in bone: a review. Archaeometry 44(3):383–394
Ferreira MT, Vicente R, Navega D, Gonçalves D, Curate F, Cunha E (2014) A new forensic collection of 21st century identified human skeletons housed at the University of Coimbra, Portugal. Forensic Sci Int 245:202.e1–202.e5. doi:10.1016/j.forsciint.2014.09.021
Carden A, Morris MD (2000) Application of vibrational spectroscopy to the study of mineralized tissues (review). J Biomed Opt 5(3):259–268. doi:10.1117%2F1.429994
Paschalis EP, Mendelsohn R, Boskey AL (2011) Infrared assessment of bone quality. Clin Orthop Relat Res 469:2170–2178. doi:10.1007/s11999-010-1751-4
Munro LE, Longstaffe FJ, White CD (2007) Burning and boiling of modern deer bone: effects on crystallinity and oxygen isotope composition of bioapatite phosphate. Palaeogeogr Palaeocl 249:90–102. doi:10.1016/j.palaeo.2007.01.011
Thompson TJU, Gauthier M, Islam M (2009) The application of a new method of Fourier transform infrared spectroscopy to the analysis of burned bone. J Archaeol Sci 36:910–914. doi:10.1016/j.jas.2008.11.013
Thompson TJU, Islam M, Bonniere M (2013) A new statistical approach for determining the crystallinity of heat-altered bone mineral from FTIR spectra. J Archaeol Sci 40:416–422. doi:10.1016/j.jas.2012.07.008
Ellingham STD, Thompson TJU, Islam M, Taylor G (2015) Estimating temperature exposure of burnt bone—a methodological review. Sci Justice. doi:10.1016/j.scijus.2014.12.002
Maspero E, Sala S, Fedi ME, Martini M, Papagni A (2011) A new procedure for extraction of collagen from modern and archaeological bones for 14C dating. Anal Bioanal Chem. doi:10.1007/s00216-011-5252-4
Grunenwald A, Keyser C, Sautereau AM, Crubézy E, Ludes B, Drouet C (2014) Revisiting carbonate quantification in apatite (bio) minerals: a validated FTIR methodology. J Archaeol Sci 49:134–141. doi:10.1016/j.jas.2014.05.004
Bellamy LJ (1975) [1954] The infrared spectra of complex molecules, vol 1, 3rd edn. Chapman and Hall, New Fetter Lane, pp 231–262
Heredia A, Colin-Garcia M, Peña-Rico MA, Aguirre Beltrán LFL, Grácio J, Contreras-Torres FF, Rodríguez-Galván A, Bucio L, Basiuk VA (2013) Thermal, infrared spectroscopy and molecular modeling characterization of bone: an insight in the apatite-collagen type I interaction. Adv Biol Chem 3:215–223. doi:10.4236/abc.2013.32027
Paschalis EP, DiCarlo E, Betts F, Sherman P, Mendelsohn R, Boskey AL (1996) FTIR microspectroscopic analysis of human osteonal bone. Calcified Tissue Int 59:480–487
Movasaghi Z, Rehman S, Rehman I (2008) Fourier transform infrared (FTIR) spectroscopy of biological tissues. Appl Spectrosc Rev 43:134–179. doi:10.1080/0570492070182904
Lebon M, Reiche I, Bahain JJ, Chadefaux C, Moigne AM, Fröhlich F, Sémah F, Schwarcz HP, Falguères C (2010) New parameters for the characterization of diagenetic alterations and heat-induced changes of fossil bone mineral using Fourier transform infrared spectrometry. J Archaeol Sci 37:265–2276. doi:10.1016/j.jas.2010.03.024
Baby RS (1954) Hopewell cremation practices. In: Papers in archaeology, vol 1. Ohio Historical Society, Columbus, pp 1–7
Bartsiokas A (2000) The eye injury of King Philip II and the skeletal evidence from the Royal Tomb II at Vergina. Science 288:511–514. doi:10.1126/science.288.5465.511
Nagy G, Lorand T, Patonai Z, Montsko G, Bajnoczky I, Marcsik A, Mark L (2008) Analysis of pathological and non-pathological human skeletal remains by FT-IR spectroscopy. Forensic Sci Int 175:55–60
Curate F (2014) Osteoporosis and paleopathology: a review. J Anthropol Sci 92:119–146
Brickley M (2002) An investigation of historical and archaeological evidence for age-related bone loss and osteoporosis. Int J Osteoarchaeol 12:364–371. doi:10.1002/oa.635
Turunen MJ, Prantner V, Jurvelin JS, Kroger H, Isaksson H (2013) Composition and microarchitecture of human trabecular bone change with age and differ between anatomical locations. Bone 54:118–125. doi:10.1016/j.bone.2013.01.045
Price TD, Blitz J, Burton J, Ezzo JA (1992) Diagenesis in prehistoric bone: problems and solutions. J Archaeol Sci 19:513–529
Acknowledgments
The authors acknowledge financial support from the Portuguese Foundation for Science and Technology – UID/MULTI/00070/2013, PTDC/IVC-ANT/1201/2014 and SFRH/BPD/84268/2012. We would also like to thank Calil Makhoul and João Coelho who helped on the preparation of the samples. We also thank Dr. Paula Marques and Ana Batista de Carvalho for their assistance in the FTIR spectra analysis.
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Vassalo, A.R., Cunha, E., de Carvalho, L.A.E.B. et al. Rather yield than break: assessing the influence of human bone collagen content on heat-induced warping through vibrational spectroscopy. Int J Legal Med 130, 1647–1656 (2016). https://doi.org/10.1007/s00414-016-1400-x
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DOI: https://doi.org/10.1007/s00414-016-1400-x